Dogra S C, Khanduja K L, Sharma R R, Hom T, Varanasi U
Biochem Int. 1985 Dec;11(6):869-84.
The metabolism of (3H)-benzo(a)pyrene and the activities of enzymes involved in its metabolism were studied in rat lung and liver in vitamin A deficiency. Deficiency of vitamin A resulted a significant decrease in the overall metabolism of benzo(a)pyrene in the liver in vitro, whereas no significant difference was evident in the lung. The ethyl acetate-soluble metabolites of benzo(a)pyrene formed by lung and liver preparations were unaltered qualitatively by vitamin A deficiency. However, quantitative analysis revealed that vitamin A deficiency decreased the yield of dihydrodiols, quinones and phenols in liver, and dihydrodiols in lung. The hepatic cytochrome P-450 content, arylhydrocarbon hydroxylase and uridine diphosphate-glucuronosyl transferase activities were reduced, whereas glutathione S-transferase activity was increased in the vitamin A deficient animals. Contrary to this, pulmonary cytochrome P-450 content was above the control values (p less than 0.01) and no alteration in pulmonary arylhydrocarbon hydroxylase activity was observed in vitamin A deficient rats. Uridine diphosphate-glucuronosyltransferase and glutathione S-transferase activities were impaired in lung by inducing vitamin A deficiency. However, no significant difference was evident in the overall metabolism of benzo(a)pyrene by lung supernatants from the two groups.
研究了维生素A缺乏的大鼠肺和肝脏中（3H）-苯并（a）芘的代谢及其代谢相关酶的活性。维生素A缺乏导致体外肝脏中苯并（a）芘的整体代谢显著下降，而肺中未观察到显著差异。肺和肝脏制剂形成的苯并（a）芘的乙酸乙酯可溶性代谢产物在质量上不受维生素A缺乏的影响。然而，定量分析表明，维生素A缺乏降低了肝脏中二氢二醇、醌和酚的产量，以及肺中二氢二醇的产量。维生素A缺乏的动物肝脏细胞色素P-450含量、芳烃羟化酶和尿苷二磷酸葡萄糖醛酸转移酶活性降低，而谷胱甘肽S-转移酶活性增加。与此相反，肺细胞色素P-450含量高于对照值（p小于0.01），维生素A缺乏的大鼠肺中芳烃羟化酶活性未观察到改变。诱导维生素A缺乏会损害肺中的尿苷二磷酸葡萄糖醛酸转移酶和谷胱甘肽S-转移酶活性。然而，两组肺上清液对苯并（a）芘的整体代谢没有明显差异。
