Department of Biological Sciences, Santa Cruz State University, Campus Soane Nazaré de Andrade, Rodovia Jorge Amado, km 16, Ilhéus, BA, Brazil.
Department of Biological Sciences, Santa Cruz State University, Campus Soane Nazaré de Andrade, Rodovia Jorge Amado, km 16, Ilhéus, BA, Brazil.
Plant Physiol Biochem. 2020 Oct;155:851-867. doi: 10.1016/j.plaphy.2020.08.039. Epub 2020 Aug 26.
Cocoa is a perennial and arboreal species intolerant to strong and frequent winds and, for this reason, is usually grown with windbreaks of trees. The mechanical alterations caused by the wind in the field have a great impact on the growth, development and productivity of cocoa. The present work had a main objective to understand the molecular mechanisms of responses to mechanical stress, caused by the action of constant wind flow in young plants of cocoa through alterations of the proteomic profile in young (YL) and mature leaves (ML). Plants were exposed to constant wind (CW) at a speed of 4.5 m s for 12 h. There was a reduction in the accumulation of proteins in YL and a significant increase in ML submitted to CW in relation to the control. Differentially accumulated proteins, identified in YL and ML, belong to a broad functional group, related to energy production and carbon metabolism. Besides that, there was a higher efficiency in the protein relative abundance associated to energy production and the assimilation of carbon in the ML exposed to CW, in relation to the control. It was observed the appearance of new isoforms and, or post-transitional changes, which represent an acclimatization and tolerance response of these leaves to the stressor factor. In contrast, in YL, the energy production and the synthesis of gene products essential for their growth and development were affected by the mechanical stress caused by the wind, making them more intolerant.
可可树是一种多年生木本植物，不耐强风和频繁的风，因此通常与防风林一起种植。田间风造成的机械改变对可可的生长、发育和生产力有很大影响。本工作的主要目的是通过研究年轻（YL）和成熟叶片（ML）中蛋白质组谱的改变，了解可可幼苗受到持续风（CW）作用引起的机械胁迫的分子响应机制，CW 的风速为 4.5 m/s，持续作用 12 小时。与对照相比，YL 中蛋白质的积累减少，而 CW 处理的 ML 中蛋白质的积累显著增加。在 YL 和 ML 中鉴定出的差异积累蛋白属于广泛的功能群，与能量产生和碳代谢有关。此外，与对照相比，CW 处理的 ML 中与能量产生和碳同化相关的蛋白质相对丰度的效率更高。观察到新的同工型和/或翻译后变化的出现，这代表了这些叶片对胁迫因子的适应和耐受反应。相比之下，YL 中的能量产生和生长发育所需的基因产物的合成受到风造成的机械胁迫的影响，使它们更不耐受。
