Institute of Soil and Water Resources and Environmental Science, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China; Hebei Province Key Laboratory of Wetland Ecology and Conservation, Hengshui University, Hengshui 053000, China.
UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
J Hazard Mater. 2021 Sep 15;418:126391. doi: 10.1016/j.jhazmat.2021.126391. Epub 2021 Jun 12.
Rare studies investigated influence of neonicotinoid insecticides on the whole soil biota including non-target invertebrates and microorganisms. And less is known about the consequent intervention on soil C processes. This study aimed to decipher Collembola-fungi-bacteria interactive effects on pathways of maize C translocation, combining isotopic tracer analysis of relevant compartments with high-throughput sequencing for bacterial and fungal genetic profiles. Dinotefuran was applied at 0 or 100 μg kg (a simulating residual dosage) to microcosms containing soils, Collembola and C labelled maize. Dinotefuran drastically reduced the density and maize-derived biomass C of Collembola, while intensifying antagonistic associations between soil organisms, with flourishing growth of Ascomycota and Actinobacteria, e.g., Streptomyces. This led to higher soil organic C (SOC) mineralization (elevated by 9.8-10.5%) across soils, attributing to the shift in microbial taxonomic and functional guild, e.g., with the increased abundance of genes aligned to cytochrome P450. Maize decomposition was controlled by Collembola that primarily fed on maize, via grazing behavior that facilitated labile maize C preferred decomposers, e.g., Xanthomonadaceae. These findings elucidate the influence of minute dinotefuran on intra-linkages between biomes (Collembola, fungi and bacteria), and highlight such legacy effects on maize and SOC mineralization.
稀有研究调查了新烟碱类杀虫剂对包括非靶标无脊椎动物和微生物在内的整个土壤生物群的影响。而对于由此对土壤 C 过程产生的后续干预则知之甚少。本研究旨在通过对相关隔室进行同位素示踪分析，并结合高通量测序进行细菌和真菌基因图谱分析，来破译跳虫-真菌-细菌对玉米 C 转移途径的相互作用。在含有土壤、跳虫和 C 标记玉米的微宇宙中，以 0 或 100μg/kg（模拟残留剂量）的浓度添加噻虫嗪。噻虫嗪大大降低了跳虫的密度和玉米衍生的生物量 C，同时加剧了土壤生物之间的拮抗关系，子囊菌门和放线菌门（例如链霉菌）大量繁殖。这导致土壤中有机碳（SOC）矿化作用增强（提高了 9.8-10.5%），这归因于微生物分类和功能类群的变化，例如细胞色素 P450 相关基因的丰度增加。玉米分解受主要以玉米为食的跳虫控制，通过放牧行为促进了易分解的玉米 C 优先分解者，例如黄单胞菌科。这些发现阐明了微量噻虫嗪对生物群（跳虫、真菌和细菌）之间内部联系的影响，并强调了这种遗留效应对玉米和 SOC 矿化的影响。
