Applied Molecular Biology Lab-LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Postgraduate Program in Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
PLoS One. 2021 Jul 30;16(7):e0255291. doi: 10.1371/journal.pone.0255291. eCollection 2021.
The diversity of the Penaeus vannamei mitochondrial genome has still been poorly characterized, there are no validated mitochondrial markers available for populational studies, and the heteroplasmy has not yet been investigated in this species. In this study, metagenomic reads extracted from the muscle of a single individual were used to assemble the mitochondrial genome (mtDNA). These data associated with mitochondrial genomes previously described allowed to evaluate the inter-individual variability and heteroplasmy. Comparison among 45 mtDNA control regions led to the detection of conserved and variable segments and the characterization of two hypervariable regions. The analysis of diversity revealed mostly low frequency polymorphisms, and heteroplasmy was found in practically all mitochondrial genes, with a high occurrence of indels. These results indicate that the design of mitochondrial markers for P. vannamei must be done with caution. The mapping of conserved and variable regions and the characterization of heteroplasmy presented here will contribute to increasing the efficiency of mitochondrial markers for population or individual studies.
凡纳滨对虾的线粒体基因组多样性仍未得到充分描述，目前尚无经过验证的线粒体标记物可用于群体研究，而且该物种的异质性尚未得到研究。在本研究中，从单个个体的肌肉中提取的宏基因组读段被用于组装线粒体基因组（mtDNA）。这些数据与之前描述的线粒体基因组相结合，用于评估个体间的变异性和异质性。对 45 个 mtDNA 控制区的比较导致了保守和可变片段的检测，并对两个高变区进行了特征描述。多样性分析显示，大多数为低频多态性，几乎所有线粒体基因都存在异质性，插入缺失的发生率很高。这些结果表明，设计凡纳滨对虾的线粒体标记物必须谨慎。这里呈现的保守和可变区的映射以及异质性的特征描述将有助于提高线粒体标记物在群体或个体研究中的效率。
