School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Anal Chim Acta. 2021 Aug 29;1175:338741. doi: 10.1016/j.aca.2021.338741. Epub 2021 Jun 7.
8-oxoguanine DNA glycosylase (OGG), which plays a crucial role in base excision repair (BER), is an important biomarker. The existing highly sensitive fluorescent methods always need complicated amplification design. The method with high sensitivity and simple design at the same time is urgently needed. Here, we developed a highly sensitive detection method for OGG detection with lambda exonuclease and the background signal suppression probe. Through probe structure design, the steric hindrance and competitive binding effects successfully suppressed the background signal. We achieved sensitive detection of OGG with a simple design, and the limit of detection was 5.0 × 10 U mL. Moreover, the method was highly selective and successfully applied to OGG detection in biological samples, which shows the potential clinical application value.
8-氧鸟嘌呤 DNA 糖基化酶（OGG）在碱基切除修复（BER）中发挥着关键作用，是一种重要的生物标志物。现有的高灵敏度荧光方法通常需要复杂的扩增设计。因此，我们迫切需要一种具有高灵敏度和简单设计的方法。在这里，我们开发了一种基于λ核酸外切酶和背景信号抑制探针的高灵敏度 OGG 检测方法。通过探针结构设计，成功利用空间位阻和竞争结合效应抑制了背景信号。我们实现了简单设计下的 OGG 灵敏检测，检测限为 5.0×10 U mL。此外，该方法具有高度选择性，并成功应用于生物样品中的 OGG 检测，显示出潜在的临床应用价值。
