激光诱导大鼠脉络膜新生血管。

Laser-Induced Choroidal Neovascularization in Rats.

机构信息

Department of Medical Physiology, College of Medicine, Texas A&M University Heath Science Center, Bryan, TX, USA.

Department of Ophthalmology, Baylor Scott & White Eye Institute, Temple, TX, USA.

出版信息

Methods Mol Biol. 2021;2319:77-85. doi: 10.1007/978-1-0716-1480-8_9.

DOI:10.1007/978-1-0716-1480-8_9

PMID:34331245

Abstract

The laser-induced choroidal neovascularization (CNV) model has been widely used for research on wet age-related macular degeneration (wet-AMD) and other ocular neovascular diseases. In this model, the Bruch membrane is perforated by laser injury, resulting in neovascularization formed from the choroidal capillaries. It has become a standard method to evaluate the effect of different treatments on CNV progression in preclinical studies. This protocol can be used in various species, including rat, mouse, pig, and monkey. The rodent laser-induced CNV model is the most commonly used because of the advantages in both cost- and time-efficiency. It takes only 10-15 min to complete the whole laser procedure after adequate training and practicing the technique. Peak CNV formation occurs at approximately 2 weeks after laser application. The entire protocol may require up to 3 weeks to complete the treatment, fundus image acquisition, and tissue collection for histologic analysis. This chapter describes the detailed procedures, protocols, and useful notes on how to induce CNV by laser.

摘要

激光诱导脉络膜新生血管（CNV）模型已广泛用于湿性年龄相关性黄斑变性（wet-AMD）和其他眼部新生血管疾病的研究。在该模型中，通过激光损伤穿透Bruch 膜，导致脉络膜毛细血管形成新生血管。它已成为评估不同治疗方法对 CNV 进展的临床前研究中的标准方法。该方案可用于包括大鼠、小鼠、猪和猴子在内的各种物种。由于成本和时间效率的优势，啮齿动物激光诱导的 CNV 模型是最常用的。在经过充分的培训和练习该技术后，整个激光手术只需 10-15 分钟即可完成。激光应用后大约 2 周达到 CNV 形成的高峰。整个方案可能需要长达 3 周的时间来完成治疗、眼底图像采集和组织收集以进行组织学分析。本章介绍了通过激光诱导 CNV 的详细过程、方案和有用说明。