Department of Medical Physiology, Texas A&M University College of Medicine, Bryan, TX, USA.
Methods Mol Biol. 2021;2319:105-110. doi: 10.1007/978-1-0716-1480-8_12.
Laser capture microdissection (LCM) enables researchers to selectively evaluate gene expression profiling of a specific cell type within a tissue. Vascular endothelial cells (EC) line the inside of vessel lumen and play an essential role in new blood vessel formation. It remains a challenge to determine vascular ECs-specific genes expression in vivo. Here, we described a method to dissect vascular ECs from the frozen heart tissue by LCM. The total RNA or proteins are then extracted from the ECs for further analysis.
激光捕获显微切割 (LCM) 可使研究人员能够选择性地评估组织中特定细胞类型的基因表达谱。血管内皮细胞 (EC) 排列在血管腔的内部，在新血管形成中起着至关重要的作用。在体内确定血管 EC 特异性基因表达仍然是一个挑战。在这里，我们描述了一种通过 LCM 从冷冻心脏组织中分离血管 EC 的方法。然后从 EC 中提取总 RNA 或蛋白质进行进一步分析。
