Potter S Steven, Brunskill Eric W
Division of Developmental Biology, Children's Hospital Medical Center, Cincinnati, OH, USA.
Methods Mol Biol. 2012;886:211-21. doi: 10.1007/978-1-61779-851-1_19.
This chapter describes detailed methods used for laser capture microdissection (LCM) of discrete subpopulations of cells. Topics covered include preparing tissue blocks, cryostat sectioning, processing slides, performing the LCM, and purification of RNA from LCM samples. Notes describe the fine points of each operation, which can often mean the difference between success and failure.
本章描述了用于对离散细胞亚群进行激光捕获显微切割（LCM）的详细方法。涵盖的主题包括制备组织块、冷冻切片、处理载玻片、进行LCM以及从LCM样品中纯化RNA。注释描述了每个操作的要点，这些要点往往决定着成败。
