近红外发射聚合物包覆的 IR-820 纳米粒子辅助光热疗法治疗宫颈癌细胞。
Near-infrared emissive polymer-coated IR-820 nanoparticles assisted photothermal therapy for cervical cancer cells.
机构信息
Department of Epidemiology and Health Statistics, School of Public Health, Xinjiang Medical University, Urumqi, China.
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, School of Medical Engineering and Technology, Xinjiang Medical University, Urumqi, China.
出版信息
J Biophotonics. 2021 Nov;14(11):e202100117. doi: 10.1002/jbio.202100117. Epub 2021 Aug 15.
Photothermal therapy (PTT) has attracted wide attention due to its noninvasiveness and its thermal ablation ability. As photothermal agents are crucial factor in PTT, those with the characteristics of biocompatibility, non-toxicity and high photothermal stability have attracted great interest. In this work, new indocyanine green (IR-820) was utilized as a photothermal agent and near-infrared (NIR) fluorescence imaging nanoprobe. To improve the biocompatibility, poly(styrene-co-maleic anhydride) (PSMA) was utilized to encapsulate the IR-820 molecules to form novel IR-820@PSMA nanoparticles (NPs). Then, the optical and thermal properties of IR-820@PSMA NPs were studied in detail. The IR-820@PSMA NPs showed excellent photothermal stability and biocompatibility. The cellular uptaking ability of the IR-820@PSMA NPs was further confirmed in HeLa cells by the NIR fluorescent confocal microscopic imaging technique. The IR-820@PSMA NPs assisted PTT of living HeLa cells was conducted under 793 nm laser excitation, and a high PTT efficiency of 73.3% was obtained.
光热疗法（PTT）由于其非侵入性和热消融能力而受到广泛关注。作为 PTT 的关键因素，那些具有生物相容性、低毒性和高热稳定性的光热剂引起了极大的兴趣。在这项工作中，新型吲哚菁绿（IR-820）被用作光热剂和近红外（NIR）荧光成像纳米探针。为了提高生物相容性，利用聚（苯乙烯-co-马来酸酐）（PSMA）将 IR-820 分子包裹形成新型 IR-820@PSMA 纳米颗粒（NPs）。然后，详细研究了 IR-820@PSMA NPs 的光学和热学性质。IR-820@PSMA NPs 表现出优异的光热稳定性和生物相容性。通过近红外荧光共聚焦显微镜成像技术进一步证实了 IR-820@PSMA NPs 在 HeLa 细胞中的细胞摄取能力。在 793nm 激光激发下，进行了活 HeLa 细胞的 IR-820@PSMA NPs 辅助 PTT，获得了 73.3%的高 PTT 效率。
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