基于定制激光系统和靶向纳米粒子的宫颈癌体内多尺度光声成像引导光热治疗。

BACKGROUND: Effective treatment strategy for cervical carcinoma is subject to the limitation of its anatomical location and histological characteristics. Comprehensive imaging before cervical carcinoma treatment is of great significance for the patients. Current imaging methods cannot meet the requirements of high resolution, deep imaging depth and non-invasive imaging at the same time. Fortunately, Photoacoustic imaging (PAI) is a novel imaging method that combines rich optical contrast, high ultrasonic spatial resolution, and deep penetration depth in a single modality. Moreover, PAI-guided photothermal therapy (PTT) by aid of targeting nanoparticles is an emerging and effective cancer treatment in recent years. METHODS: Here, strong near-infrared region (NIR) absorption-conjugated polymer PIIGDTS (PD) nanoparticles with folic acid (FA) modification (namely, PD-FA) that targeted at Hela cell were specifically designed as cervical tumor imaging contrast agents and photothermal agents. RESULTS: The obtained PD-FA nanoparticles exhibited admirable photoacoustic contrast-enhancing ability and desirable PTT behavior with the photothermal conversion efficiency as high as 62.6% in vitro. Furthermore, the PAI performance and PTT efficiency were tested in HeLa tumor-bearing nude mice after injection of PD-FA nanoparticles. In vivo multi-scale, PAI provided B-san and 3D dimension imaging for intuitive and comprehensive information of Hela tumor. Moreover, the Hela tumor can be completely eliminated within 18 days after PTT, with no toxicity and side effects. CONCLUSION: In summary, PD-FA injection combined with PAI and PTT systems provides a novel powerful tool for early diagnosis and precise treatment of cervical cancer.

背景：宫颈癌的有效治疗策略受到其解剖位置和组织学特征的限制。宫颈癌治疗前的综合影像学检查对患者具有重要意义。目前的成像方法不能同时满足高分辨率、深成像深度和非侵入性成像的要求。幸运的是，光声成像是一种将丰富的光学对比度、高超声空间分辨率和单一模态下的深穿透深度相结合的新型成像方法。此外，借助靶向纳米颗粒的光声引导光热疗法（PTT）是近年来新兴的有效癌症治疗方法。

方法：在这里，我们专门设计了具有叶酸（FA）修饰的强近红外（NIR）吸收共轭聚合物 PIIGDTS（PD）纳米粒子（即 PD-FA）作为宫颈癌肿瘤成像对比剂和光热剂。

结果：所得到的 PD-FA 纳米粒子表现出令人钦佩的光声对比增强能力和理想的 PTT 行为，其光热转换效率高达 62.6%。此外，在注射 PD-FA 纳米粒子后，我们在荷瘤裸鼠中测试了 PAI 性能和 PTT 效率。体内多尺度 PAI 为 Hela 肿瘤提供了直观和全面的 B 型和 3D 维度成像信息。此外，在 PTT 后 18 天内，Hela 肿瘤可以完全消除，没有毒性和副作用。

结论：综上所述，PD-FA 注射联合 PAI 和 PTT 系统为宫颈癌的早期诊断和精确治疗提供了一种新的强大工具。