Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Lasers Med Sci. 2021 Dec;36(9):1971-1977. doi: 10.1007/s10103-021-03389-9. Epub 2021 Jul 31.
Antimicrobial photodynamic therapy (aPDT) is an alternative approach. The current study aimed to investigate the efficacy of aPDT with indocyanine green (ICG) against two Candida albicans (C. albicans) strains. In this in vitro study, the inoculum of standard ATCC 10,231 (S) and fluconazole-resistant (FR) strains were adjusted to the turbidity of a 0.5 McFarland standard. Each strain was allocated into 4 groups: S1 and FR1) control groups, S2 and FR2) ICG-treated groups (1 µg/mL), S3 and FR3) laser-irradiated groups (wavelength: 810 nm; mode: continuous-wave; output power: 300 mW; spot size: 4.5 mm; exposure time: 120 s; radiation dose: 228 J/cm), S4 and FR4) ICG-mediated-aPDT groups. After treatments, the number of colony-forming units per milliliter (CFU/mL) was calculated. Using the XTT reduction assay, the effects of each treatment on Candida biofilm formation were evaluated. Data were analyzed using SPSS software version 22. In both strains, the maximum number of CFUs was observed in the control group, followed by ICG-treated, laser-irradiated, and ICG-mediated-aPDT groups. In ATCC 10,231 strain, the XTT assay exhibited significant difference between ICG-mediated-aPDT and control groups (p < 0.0001). However, the ICG, laser, and ICG-mediated-aPDT groups in fluconazole-resistant strain showed significant differences when compared with the control (p < 0.05). The mean Candida CFUs and the XTT assay did not show any significant difference between the ATCC 10,231 and fluconazole-resistant strains with respect to each treatment. Data suggest ICG-mediated-aPDT could diminish Candida CFUs in laboratory; however, further studies are warranted to confirm its efficacy and safety to be applied in clinics.
抗菌光动力疗法（aPDT）是一种替代方法。本研究旨在探讨使用吲哚菁绿（ICG）进行 aPDT 对两种白色念珠菌（C. albicans）菌株的疗效。在这项体外研究中，将标准 ATCC 10,231（S）和氟康唑耐药（FR）菌株的接种物调整为 0.5 McFarland 标准浊度。每个菌株分为 4 组：S1 和 FR1）对照组、S2 和 FR2）ICG 处理组（1μg/mL）、S3 和 FR3）激光照射组（波长：810nm；模式：连续波；输出功率：300mW；光斑大小：4.5mm；照射时间：120s；辐射剂量：228J/cm）、S4 和 FR4）ICG 介导的-aPDT 组。处理后，计算每毫升菌落形成单位（CFU/mL）的数量。使用 XTT 还原测定法评估每种处理方法对念珠菌生物膜形成的影响。使用 SPSS 软件版本 22 分析数据。在两种菌株中，对照组观察到的 CFU 数量最多，其次是 ICG 处理组、激光照射组和 ICG 介导的-aPDT 组。在 ATCC 10,231 菌株中，XTT 测定法显示 ICG 介导的-aPDT 与对照组之间存在显著差异（p<0.0001）。然而，与对照组相比，氟康唑耐药株的 ICG、激光和 ICG 介导的-aPDT 组显示出显著差异（p<0.05）。就每种处理方法而言，ATCC 10,231 和氟康唑耐药菌株的念珠菌 CFU 和 XTT 测定法之间没有显示出任何显著差异。数据表明，ICG 介导的-aPDT 可以减少实验室中的念珠菌 CFU；然而，需要进一步的研究来确认其在临床上的疗效和安全性。
