Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Cytokine. 2021 Oct;146:155643. doi: 10.1016/j.cyto.2021.155643. Epub 2021 Jul 28.
To compare mRNA expression of interleukin 10 (IL-10), interleukin 17 (IL-17) and Transforming Growth Factor-β (TGF-β) in aqueous humor (AH) and peripheral blood mononuclear cells (PBMCs) in human ocular toxoplasmosis (OT) and controls.
RNA isolation, cDNA synthesis and real-time polymerase chain reaction were performed on AH sediments and PBMCs of 16 patients with active OT and 21 controls at the Khatam-al-Anbia Eye Hospital, Iran. For comparison, Mann Whitney U test was used at a discrimination level of p < 0.05. Pearson and Spearman rank correlation test were applied for correlation with clinical parameters.
The expression for IL-10 and IL-17 in the AH was 3.7- and 88.0-fold higher in OT than in controls (P = 0.04 and P = 0.03, respectively) whereas that of TGF-β was 7.7-fold lower (P < 0.001). The expression levels for these cytokines in PBMC followed a similar pattern (IL-10 13.8-fold down-regulated (P = 0.001), IL-17 with 1.9-fold insignificantly upregulated (p = 0.43), TGF-β 452.8-fold down-regulated (P = 0.002). Compared to PBMC, IL-10 coding mRNA was 1876-fold higher in the almost cell-free AH in OT (39.2-fold in controls), IL-17 coding mRNA was 9.4-fold higher (17.7-fold down-regulated in controls), and that coding for TGF-β 207-fold higher in OT (7x10-fold in controls). The expression for IL-10, IL-17 and TGF-β in AH thus followed an opposite pattern compared to that in PBMC.
OT induces a highly-specific local immunoregulatory process as evidenced by an intraocular up-regulation of IL-10 and down-regulation of TGF-β mRNA. This could indicate an attempt to prevent unnecessary tissue damage which is in line with a moderate local mRNA up-regulation for IL-17 which seems sufficient to control parasite proliferation. That this regulation is opposite to that in PBMC may be linked to intraocular immune deviation in the course of disease.
比较人眼弓形体病（OT）患者和对照组房水（AH）和外周血单个核细胞（PBMC）中白细胞介素 10（IL-10）、白细胞介素 17（IL-17）和转化生长因子-β（TGF-β）的 mRNA 表达。
在伊朗 Khatam-al-Anbia 眼科医院，对 16 例活动性 OT 患者和 21 例对照组的 AH 沉淀物和 PBMC 进行 RNA 分离、cDNA 合成和实时聚合酶链反应。为了进行比较，采用 Mann Whitney U 检验，差异水平为 p < 0.05。采用 Pearson 和 Spearman 秩相关检验与临床参数进行相关性分析。
OT 患者的 AH 中 IL-10 和 IL-17 的表达分别比对照组高 3.7 倍和 88.0 倍（P=0.04 和 P=0.03），而 TGF-β 的表达则低 7.7 倍（P<0.001）。这些细胞因子在 PBMC 中的表达水平也呈现出类似的模式（IL-10 下调 13.8 倍（P=0.001），IL-17 上调 1.9 倍，但无统计学意义（p=0.43），TGF-β 下调 452.8 倍（P=0.002）。与 PBMC 相比，OT 患者的几乎无细胞 AH 中 IL-10 编码 mRNA 高 1876 倍（对照组中为 39.2 倍），IL-17 编码 mRNA 高 9.4 倍（对照组中为 17.7 倍下调），TGF-β 编码 mRNA 高 207 倍（对照组中为 7x10 倍）。因此，AH 中 IL-10、IL-17 和 TGF-β 的表达与 PBMC 中的表达相反。
OT 诱导一种高度特异性的局部免疫调节过程，表现为眼内 IL-10 上调和 TGF-β mRNA 下调。这可能表明一种试图防止不必要的组织损伤的尝试，这与 IL-17 的局部 mRNA 适度上调一致，这似乎足以控制寄生虫增殖。这种调节与 PBMC 中的调节相反，可能与疾病过程中的眼内免疫偏离有关。
