Maia M M, Meira-Strejevitch C S, Pereira-Chioccola V L, de Hippólito D D C, Silva V O, Brandão de Mattos C C, Frederico F B, Siqueira R C, de Mattos L C
Centro de Parasitologia e Micologia, Instituto Adolfo Lutz, Sao Paulo, Brazil.
Laboratório de Imunogenética, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brazil.
Parasite Immunol. 2017 Oct;39(10). doi: 10.1111/pim.12462.
This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) of patients with ocular toxoplasmosis (OT Group, n = 23) and chronic toxoplasmosis individuals (CHR Group, n = 9) were isolated and stimulated in vitro with T. gondii antigen. Negative controls (NC) were constituted of 7 PBMC samples from individuals seronegative for toxoplasmosis. mRNA expression for cytokines was determined by qPCR. Results showed a significant increase in mRNA levels from antigen stimulated PBMCs derived from OT Group for expressing IL-6 (at P < .005 and P < .0005 for CHR and NC groups, respectively), IL-10 (at P < .0005 and P < .005 for CHR and NC groups, respectively) and TGF-β (at P < .005) for NC group. mRNA levels for TNF-α and IL-12 were also upregulated in patients with OT compared to CHR and NC individuals, although without statistical significance. Additionally, mRNA levels for IL-27 and IFN-γ in PBMC of patients with OT were upregulated in comparison with NC individuals. Differences between OT and NC groups were statistically significant at P < .05 and P < .0005, respectively.
本研究评估了眼部弓形虫病中7种细胞因子的mRNA表达水平。分离出眼部弓形虫病患者（OT组，n = 23）和慢性弓形虫病患者（CHR组，n = 9）的外周血单个核细胞（PBMC），并在体外用弓形虫抗原进行刺激。阴性对照（NC）由来自弓形虫病血清阴性个体的7个PBMC样本组成。通过qPCR测定细胞因子的mRNA表达。结果显示，OT组抗原刺激的PBMC中，IL-6（CHR组和NC组分别为P <.005和P <.0005）、IL-10（CHR组和NC组分别为P <.0005和P <.005）和TGF-β（NC组为P <.005）的mRNA水平显著升高。与CHR组和NC组个体相比，OT患者中TNF-α和IL-12的mRNA水平也上调，尽管无统计学意义。此外，与NC个体相比，OT患者PBMC中IL-27和IFN-γ的mRNA水平上调。OT组与NC组之间的差异分别在P <.05和P <.0005时具有统计学意义。
