State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
Beijing Enterprise of Technology Service (Guangdong) Co.LTD., Guangzhou 510360, China.
Bioresour Technol. 2021 Nov;340:125582. doi: 10.1016/j.biortech.2021.125582. Epub 2021 Jul 16.
The study aimed to isolate a novel strain with heterotrophic nitrification and aerobic denitrification ability and evaluate the nitrogen removal characteristics. Results showed that Ochrobactrum anthropi HND19 could remove approximately 98.6% of NH-N (104.3 mg·L) and 97.6% of NO-N (98.6 mg·L), and the removal rates achieved 4.28 and 4.01 mg-N/(L·h) by heterotrophic nitrification and aerobic denitrification. The optimal incubate conditions of strain HND19 were 120 rpm (shaking speed), 5 ‰ (salinity), 30 °C (temperature), 7.5 (C/N ratio) with sodium acetate as carbon resource. And the removal efficiency of the total nitrogen (TN) realized 73.4% under the optimal conditions. Functional genes (hao, napA, nirK, norB, and nosZ) involved in the nitrogen removal processes were successfully amplified from strain HND19. These findings indicate that the strain HND19 possesses great application feasibility in treating wastewater with high-intensity nitrogen.
本研究旨在分离具有异养硝化和好氧反硝化能力的新型菌株，并评估其脱氮特性。结果表明，Ochrobactrum anthropi HND19 可以去除约 98.6%的 NH-N（104.3mg·L）和 97.6%的 NO-N（98.6mg·L），通过异养硝化和好氧反硝化，其脱氮速率分别达到 4.28 和 4.01mg-N/（L·h）。菌株 HND19 的最佳培养条件为 120rpm（摇床速度）、5‰（盐度）、30°C（温度）、7.5（C/N 比），以乙酸钠作为碳源。在最佳条件下，总氮（TN）的去除效率达到 73.4%。成功从菌株 HND19 中扩增出参与脱氮过程的功能基因（hao、napA、nirK、norB 和 nosZ）。这些发现表明，菌株 HND19 在处理高强度氮废水方面具有很大的应用可行性。
