Wang Bingguo, Peng Huanlong, Liu Wei
School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510006, China.
Microorganisms. 2024 Dec 20;12(12):2652. doi: 10.3390/microorganisms12122652.
A novel bacterial strain, DGFC5, was isolated from a municipal sewage disposal system. It efficiently removed ammonium, nitrate, and nitrite under conditions of 5% salinity, without intermediate accumulation. Provided with a mixed nitrogen source, DGFC5 showed a higher utilization priority for NH-N. Whole-genome sequencing and nitrogen balance experiments revealed that DGFC5 can simultaneously consume NH-N in the liquid phase through assimilation and heterotrophic nitrification, and effectively remove nitrate via aerobic denitrification and dissimilatory reduction reactions. Single-factor experiments were conducted to determine the optimal nitrogen removal conditions, which were as follows: a carbon-to-nitrogen ratio of 15, a shaking speed of 200 rpm, a pH of 7, CHNaO as the carbon source, and a temperature of 30 °C. DGFC5 showed efficient nitrogen purification capabilities under a wide range of environmental conditions, indicating its potential for disposing of nitrogenous wastewater with high salinity.
从城市污水处理系统中分离出一种新型细菌菌株DGFC5。在5%盐度条件下，它能有效去除铵、硝酸盐和亚硝酸盐，且无中间产物积累。在提供混合氮源的情况下，DGFC5对NH-N表现出更高的利用优先级。全基因组测序和氮平衡实验表明，DGFC5可通过同化作用和异养硝化作用同时消耗液相中的NH-N，并通过好氧反硝化作用和异化还原反应有效去除硝酸盐。进行单因素实验以确定最佳脱氮条件，具体如下：碳氮比为15，振荡速度为200 rpm，pH值为7，以CHNaO作为碳源，温度为30℃。DGFC5在广泛的环境条件下均表现出高效的氮净化能力，表明其在处理高盐度含氮废水方面具有潜力。
