Seguin M C, Harrison D N, Williams J E
Department of Hazardous Microorganisms, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Toxicon. 1987;25(9):989-94. doi: 10.1016/0041-0101(87)90162-0.
The murine toxins A and B of Y. pestis were purified from a crude extract of the nonencapsulated strain M23NP of Y. pestis using HPLC gel filtration. Toxins A and B had molecular weights of 240,000 and 120,000, respectively. Both toxins had isoelectric points of 3.8. The mouse LD50 of toxin A was 2.5 micrograms, that of toxin B was 1.8 micrograms. Of the protein injected on the column, approximately 9% was toxin, including 2.9% toxin A and 6.1% toxin B. The crude M23NP extract was used periodically over 10 months to purify lots of the toxins. Each HPLC purification required about 30 min.
利用高效液相色谱凝胶过滤法，从鼠疫耶尔森氏菌非荚膜菌株M23NP的粗提物中纯化出鼠疫耶尔森氏菌的鼠毒素A和B。毒素A和B的分子量分别为240,000和120,000。两种毒素的等电点均为3.8。毒素A对小鼠的半数致死量为2.5微克，毒素B为1.8微克。注入柱中的蛋白质约9%为毒素，其中包括2.9%的毒素A和6.1%的毒素B。在10个月的时间里定期使用M23NP粗提物来纯化大量毒素。每次高效液相色谱纯化大约需要30分钟。
