Department of Agriculture, Food and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche 10, Ancona 60131, Italy.
Department of Agriculture, Food and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche 10, Ancona 60131, Italy.
Bioresour Technol. 2021 Nov;340:125662. doi: 10.1016/j.biortech.2021.125662. Epub 2021 Jul 24.
Post-digestate treatments may reduce the risk linked to Antibiotic Resistant Genes (ARGs) release with digestate direct land application. Thus, this study aimed to evaluate post-digestate composting and co-composting with biogas production feedstock (maize silage, food processing waste, and poultry litter) effect on abundance of selected ARGs: erm(B), tet(K), tet(M), tet(O), and tet(S) genes. More than 80% of all ARGs were removed after 90 days of composting but removals from co-composting were lower. Bacteroidetes, Firmicutes, and Proteobacteria dominated fresh digestate, and a network analysis indicated that these were potential hosts of ARGs. The emergence of Actinobacteria (dominant), Planctomycetes, and Verrucomicrobia phyla during composting shifted the microbial composition. Moreover, canonical correspondence analysis showed trace elements explaining 90% variations in ARGs abundance. The study illustrates significance of post-digestate composting in mitigating ARGs release, and effectiveness could be linked to shift in microbial composition and trace elements release.
后消化处理可降低消化物直接土地应用中与抗生素抗性基因（ARGs）释放相关的风险。因此，本研究旨在评估后消化堆肥和与沼气生产原料（玉米青贮、食品加工废物和家禽粪便）共堆肥对选定 ARGs（erm(B)、tet(K)、tet(M)、tet(O)和 tet(S)基因）丰度的影响。在堆肥 90 天后，超过 80%的所有 ARGs 被去除，但共堆肥的去除率较低。拟杆菌门、厚壁菌门和变形菌门在新鲜消化物中占主导地位，网络分析表明这些是 ARGs 的潜在宿主。在堆肥过程中，放线菌门（优势）、浮霉菌门和疣微菌门的出现改变了微生物组成。此外，典范对应分析表明痕量元素解释了 ARGs 丰度变化的 90%。该研究说明了后消化堆肥在减轻 ARGs 释放方面的重要性，其效果可能与微生物组成和痕量元素释放的变化有关。
