Bioengineering and Environmental Sciences (BEES) Group, Department of Energy and Environmental Engineering (DEEE), CSIR-Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad 500007, India; Academy of Scientific and Industrial Research (AcSIR), Ghaziabad, 201002, India.
Bioengineering and Environmental Sciences (BEES) Group, Department of Energy and Environmental Engineering (DEEE), CSIR-Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad 500007, India.
Bioresour Technol. 2021 Nov;340:125675. doi: 10.1016/j.biortech.2021.125675. Epub 2021 Jul 27.
This study investigated the impact of the potential cellulose degrading bacteria that could be bioaugmented in the solid-state anaerobic digestion (SSAD) of bagasse to enhance the methane yield. The prospective anaerobic cellulose degrading bacteria was isolated from the soil. SSAD experiments were organized with & without bioaugmentation with a substrate total solid (TS) of 25%, 30%, 40% and 50% at an optimized feed to microorganism (F/M) ratio of 1:1. The maximum yield of 0.44 L CH/ (g VS added) was obtained from bioaugmented bagasse at a TS of 40% whereas it was 0.34 L CH/(g VS added) for non-bioaugmented bagasse. The isolated bacterial strain was identified that belongs species Pseudomonas of Gamma Proteobacteria which exhibited good cellulolytic activity. Metagenomic studies found 90% of archaeal microorganisms affiliated to Methanosaeta, a strict acetoclastic methanogen.
本研究调查了在甘蔗渣固态厌氧消化（SSAD）中可能进行生物强化的潜在纤维素降解细菌对提高甲烷产量的影响。从土壤中分离出潜在的厌氧纤维素降解细菌。在优化的进料与微生物（F/M）比为 1:1 的条件下，用总固体（TS）为 25%、30%、40%和 50%的底物进行 SSAD 实验，不进行生物强化和进行生物强化。在 TS 为 40%的情况下，用生物强化的甘蔗渣获得了 0.44 L CH/（g 添加的 VS）的最大产量，而未经生物强化的甘蔗渣的产量为 0.34 L CH/（g 添加的 VS）。分离出的细菌菌株被鉴定为属于γ变形菌门的假单胞菌，具有良好的纤维素酶活性。宏基因组研究发现，90%的古菌微生物与产甲烷菌 Methanosaeta 有关，产甲烷菌 Methanosaeta 是一种严格的乙酸营养型产甲烷菌。
