Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, João Dagnone Avenue, 1100, CEP, 13563-120, São Carlos, SP, Brazil.
Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, João Dagnone Avenue, 1100, CEP, 13563-120, São Carlos, SP, Brazil.
Enzyme Microb Technol. 2023 Jan;162:110119. doi: 10.1016/j.enzmictec.2022.110119. Epub 2022 Sep 5.
Enzymatically pre-treated sugarcane bagasse (SCB) was used as substrate for sequential production of hydrogen (H) and methane (CH). In stage I, SCB was used by autochthonous bacteria, such as Enterococcus (42.4 % relative abundance) and Paraclostridium (16.8 %) for H production (166.8 mL H/L), while the metabolites accumulated at the end of this stage (9140.5 mg HAc/L) were used by allochthonous inoculum for CH production (870.8 mL CH/L) in stage II. In stage II, hydrogenotrophic (Methanoculleus, 49.1 %) and acetoclastic (Methanosaeta, 15.5 %) archaeal genera were identified and considered important to maintain low H pressure in the system. According to gene inference, the hydrolysis of the SCB fiber was performed in both stages, as potential β-glucosidase and 1,4-β-xylosidase encoding genes were predicted. However, the energy metabolism of microbial populations differed, as potential genes involved in CH metabolism were predominant in phase II (39.0 %).
用酶预处理过的甘蔗渣（SCB）作为底物，连续生产氢气（H）和甲烷（CH）。在第一阶段，肠球菌（相对丰度 42.4%）和拟杆菌（Paraclostridium）（16.8%）等土著细菌用于 H 生产（166.8 mL H/L），而在此阶段结束时积累的代谢产物（9140.5 mg HAc/L）被异源接种物用于 CH 生产（870.8 mL CH/L）在第二阶段。在第二阶段，鉴定出氢营养型（Methanoculleus，49.1%）和乙酰营养型（Methanosaeta，15.5%）古菌属，它们被认为对维持系统中低 H 压力很重要。根据基因推断，在两个阶段都进行了 SCB 纤维的水解，因为预测到了潜在的β-葡萄糖苷酶和 1,4-β-木聚糖酶编码基因。然而，微生物种群的能量代谢不同，因为 CH 代谢相关的潜在基因在第二阶段占主导地位（39.0%）。
