Anand Anjali, Chatterjee Bandhan, Dhiman Abhijeet, Goel Renu, Khan Eshan, Malhotra Anita, Santra Vishal, Salvi Nitin, Khadilkar M V, Bhatnagar Ira, Kumar Amit, Asthana Amit, Sharma Tarun Kumar
Aptamer Technology and Diagnostics Laboratory, Multidisciplinary Clinical and Translational Research Group, Translational Health Science and Technology Institute (THSTI), Faridabad, Haryana, 121001, India.
Translational Health Science and Technology Institute (THSTI), Faridabad, Haryana, 121001, India.
Biosens Bioelectron. 2021 Dec 1;193:113523. doi: 10.1016/j.bios.2021.113523. Epub 2021 Jul 24.
Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is β-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.
复杂靶标SELEX一直是科学界感兴趣的一种方法，因为它有可能发现新的生物标志物。我们在此成功地对眼镜蛇毒进行了SELEX，以开发一组高度亲和的适体，这些适体能够特异性识别眼镜蛇（普通金环蛇）毒液，并能够将眼镜蛇毒液与眼镜王蛇、罗素蝰蛇和锯鳞蝰蛇的毒液区分开来。针对粗毒液产生的适体还导致了毒液特定成分的鉴定，即β-银环蛇毒素，这是一种仅存在于眼镜蛇毒液中的毒素。性能最佳的适体候选物被用作纸质装置中的分子识别元件，能够在人血清背景中检测低至2 ng的金环蛇毒液。所开发的基于适体的纸质装置因其简单性和经济性，可用于潜在的即时毒液检测应用。
