Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.
Department of Urology, West China Hospital, Sichuan University, Chengdu, China.
Andrology. 2022 Jan;10(1):143-153. doi: 10.1111/andr.13085. Epub 2021 Aug 13.
Intracavernous pressure measurement following cavernous nerve electrostimulation has been extensively adopted for the evaluation of erectile function in animals. However, the effect of measurement time and acidosis during anesthesia is still lacking.
To explore the effect of measurement time and acidosis during anesthesia.
Fifty-six male Sprague-Dawley rats were used and anesthetized by a spontaneous inhalation of isoflurane. In the first step, rats were randomly divided into four groups: a control group and three time-delayed measurement groups (intracavernous pressure measurement beginning at 15, 30, and 45 min after cavernous nerve exposure). In the second step, rats were randomly divided into three groups: a control group and two time-delayed measurement groups. Two intravenous fluid support strategies were used in time-delayed measurement groups: a normal saline solution and an isotonic Na CO solution.
Isoflurane-anesthetized rats developed systemic acidosis that worsens with time during intracavernous pressure measurement, which results in a significant decrease in the maximum intracavernous pressure value, intracavernous pressure/mean arterial pressure ratio, and total intracavernous pressure measured. The Na CO infusion could effectively correct acidosis. The decrease in intracavernous pressure was related to the reduced nitric oxide synthase activity, decreased cyclic guanosine monophosphate concentration, and reactive oxygen species activation in rat penis under acidosis conditions.
Prolonged isoflurane anesthesia-induced acidosis markedly depresses the erectile response to cavernous nerve electrostimulation in rats. In this situation, it is recommended to supplement with a Na CO infusion to maintain a normal acid-base balance.
海绵体内压测量在神经电刺激后被广泛应用于动物的勃起功能评估。然而，麻醉过程中测量时间和酸中毒的影响仍不清楚。
探讨测量时间和酸中毒对勃起功能的影响。
使用 56 只雄性 Sprague-Dawley 大鼠，通过吸入异氟烷实现自发麻醉。第一步，大鼠随机分为四组：对照组和三个延时测量组（海绵体神经暴露后 15、30 和 45 分钟开始进行海绵体内压测量）。第二步，大鼠随机分为三组：对照组和两个延时测量组。在延时测量组中，采用两种静脉输液支持策略：生理盐水和等渗 NaHCO3 溶液。
异氟烷麻醉大鼠在进行海绵体内压测量时会发生全身酸中毒，且随着时间的推移而加重，导致最大海绵体内压值、海绵体内压/平均动脉压比值和总海绵体内压测量值显著降低。NaHCO3 输注可有效纠正酸中毒。在酸中毒条件下，大鼠阴茎中一氧化氮合酶活性降低、环鸟苷酸浓度降低和活性氧物质激活，与海绵体内压降低有关。
长时间异氟烷麻醉引起的酸中毒显著抑制了大鼠海绵体神经电刺激的勃起反应。在这种情况下，建议补充 NaHCO3 输注以维持正常的酸碱平衡。
