Hotta Yuji, Shiota Arufumi, Kataoka Tomoya, Motonari Marina, Maeda Yasuhiro, Morita Masahiko, Kimura Kazunori
Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Int J Urol. 2014 Jun;21(6):608-12. doi: 10.1111/iju.12362. Epub 2013 Dec 23.
To investigate the efficacy of oral L-citrulline for erectile dysfunction and penile structure disruption in a rat model.
Male Wistar-ST rats aged 15 weeks were randomly divided into three groups as follows: sham-operated rats (control group), surgically castrated rats (castrated group) and surgically castrated rats subsequently treated with 2% L-citrulline water (castrated + L-citrulline). At 4 weeks postoperative, erectile function was assessed based on intracavernous pressure changes, followed by electrostimulation of cavernous nerves and calculation of maximum intracavernous pressure/mean arterial pressure. Penile structure was evaluated by Masson's trichrome staining and the smooth muscle-to-collagen ratio was calculated. The serum bioavailable testosterone, L-arginine, L-citrulline, N(G),N(G) -dimethylarginine and nitrogen oxide levels were evaluated.
The bioavailable testosterone concentrations were decreased in the castrated and castrated + L-citrulline groups compared with the control group at 4 weeks after surgery. The intracavernous pressure-to-mean arterial pressure and smooth muscle-to-collagen ratios were significantly decreased in the castrated group compared with the control group, but significantly increased in the castrated + L-citrulline group compared with the castrated group. The serum L-citrulline, L-arginine and N(G),N(G)-dimethylarginine levels, and the L-arginine-to-N(G),N(G)-dimethylarginine ratios were significantly increased in the castrated +L-citrulline group compared with the castrated group. The serum nitrogen oxide levels were increased in the castrated + L-citrulline group compared with the castrated group.
Oral L-citrulline can improve the erectile response to electric stimulation of cavernous nerve and penile structure in castrated rats.
在大鼠模型中研究口服L-瓜氨酸对勃起功能障碍和阴茎结构破坏的疗效。
将15周龄的雄性Wistar-ST大鼠随机分为三组：假手术大鼠（对照组）、手术去势大鼠（去势组）和手术去势后用2%L-瓜氨酸水治疗的大鼠（去势+L-瓜氨酸组）。术后4周，根据海绵体内压力变化评估勃起功能，随后进行海绵体神经电刺激并计算最大海绵体内压力/平均动脉压。通过Masson三色染色评估阴茎结构并计算平滑肌与胶原比例。评估血清中生物可利用睾酮、L-精氨酸、L-瓜氨酸、N(G),N(G)-二甲基精氨酸和一氧化氮水平。
与对照组相比，去势组和去势+L-瓜氨酸组术后4周生物可利用睾酮浓度降低。与对照组相比，去势组海绵体内压力与平均动脉压以及平滑肌与胶原比例显著降低，但与去势组相比，去势+L-瓜氨酸组显著升高。与去势组相比，去势+L-瓜氨酸组血清L-瓜氨酸、L-精氨酸和N(G),N(G)-二甲基精氨酸水平以及L-精氨酸与N(G),N(G)-二甲基精氨酸比例显著升高。与去势组相比，去势+L-瓜氨酸组血清一氧化氮水平升高。
口服L-瓜氨酸可改善去势大鼠对海绵体神经电刺激的勃起反应和阴茎结构。
