BioTechnology Institute, University of Minnesota, St. Paul, MN, USA.
Department of Earth and Environmental Sciences, University of Minnesota, Minneapolis, MN, USA.
Int J Phytoremediation. 2022;24(4):420-428. doi: 10.1080/15226514.2021.1951654. Epub 2021 Aug 1.
Inorganic arsenic (As) is a toxic and carcinogenic pollutant that has long-term impacts on environmental quality and human health. plants hyperaccumulate As from soils. Soil bacteria are critical for As-uptake by . We examined the use of taxonomically diverse soil bacteria to modulate As speciation in soil and their effect on As-uptake by . Aqueous media inoculated with MK800041, MK344656, MK345459, MK346993 or MK346997 resulted in the oxidation of 5-30% As(III) and a 49-79% reduction of As(V). Soil inoculated with increased extractable As(III) and As(V) from 0.5 and 0.09 in controls to 0.9 and 0.39 mg As kg soil dry weight, respectively. Moreover, and plants inoculated with , , strains, and strains MK344655, MK346994, MK346997, significantly increased As-uptake by 43, 32, 12, 18, 16, and 14%, respectively, compared to controls. The greatest As-accumulation (1.9 ± 0.04 g kg frond Dwt) and bioconcentration factor (16.3 ± 0.35) was achieved in plants inoculated with . Our findings indicate that the tested bacterial strains can increase As-availability in soils, thus enhancing As-accumulation by . , a well-known As-hyperaccumulator, has the remarkable ability to accumulate higher levels of As in their above-ground biomass. The As-tolerant bacteria-plant interactions play a significant role in bioremediation by mediating As-redox and controlling As-availability and uptake by . Our studies indicated that most of the tested bacterial strains isolated from As-impacted soil significantly enhanced As-uptake by . oxidized 20% of As(III) and reduced 50% of As(V), increased As-extraction from soils, and increased As-uptake by 43% greater compared with control. Therefore, these strains associated with can be used in large-scale field applications to remediate As-contaminated soil.
无机砷 (As) 是一种有毒致癌的污染物，对环境质量和人类健康有着长期的影响。超积累植物从土壤中吸收砷。土壤细菌对植物吸收砷至关重要。我们研究了利用分类多样的土壤细菌来调节土壤中砷的形态，以及它们对植物吸收砷的影响。用 MK800041、MK344656、MK345459、MK346993 或 MK346997 水培的细菌使 5-30%的 As(III)氧化，并使 As(V)减少 49-79%。与对照相比，用 接种的土壤中可提取的 As(III)和 As(V)分别从 0.5 和 0.09 增加到 0.9 和 0.39 mg As kg 土壤干重。此外，与对照相比，用 、 、 、 、 、 菌株接种的 植物对砷的吸收分别增加了 43%、32%、12%、18%、16%和 14%。接种 菌株的植物砷积累量最大（1.9±0.04 g kg 叶柄干重）和生物浓缩因子（16.3±0.35）最高。我们的研究结果表明，所测试的细菌菌株可以增加土壤中砷的有效性，从而增强 植物对砷的积累。作为一种众所周知的砷超积累植物， 具有在地上生物量中积累更高水平砷的显著能力。砷耐受细菌-植物相互作用通过调节砷的氧化还原和控制砷的有效性和吸收，在生物修复中起着重要作用。我们的研究表明，从砷污染土壤中分离出的大多数细菌菌株显著增强了 植物对砷的吸收。 氧化 20%的 As(III)，还原 50%的 As(V)，增加土壤中砷的提取量，并使砷的吸收增加 43%。因此，这些与 相关的菌株可以用于大规模的田间应用，以修复砷污染的土壤。
