Antenozio Maria Luisa, Giannelli Gianluigi, Fragni Rosaria, Baragaño Diego, Brunetti Patrizia, Visioli Giovanna, Cardarelli Maura
IBPM-CNR c/o Dipartimento di Biologia e Biotecnologie, Sapienza Università di Roma, Piazzale Aldo Moro, 00185 Roma, Italy.
Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze 11/A, 43124 Parma, Italy.
Plants (Basel). 2024 Jul 24;13(15):2030. doi: 10.3390/plants13152030.
This greenhouse study evaluated the effects of soil enrichment with rhizosphere bacteria on the growth and accumulation of arsenic in grown on a naturally As-rich soil. Inoculations were performed with a consortium of six bacteria resistant to 100 mM arsenate and effects were compared to those obtained on the sterilized soil. Selected bacteria from the consortium were also utilized individually: PVr_9 homologous to that produces IAA and siderophores and shows ACC deaminase activity, PVr_15 homologous to that contains the arsenate reductase gene, and PVr_5 homologous to that possesses all traits from both PVr_9 and PVr_15. Frond and root biomass significantly increased in ferns inoculated with the consortium only on non-sterilized soil. A greater increase was obtained with PVr_9 alone, while only an increased root length was found in those inoculated with either PVr_5 or PVr_15. Arsenic content significantly decreased only in ferns inoculated with PVr_9 while it increased in those inoculated with PVr_5 and PVr_15. In conclusion, inoculations with the consortium and PVr_9 alone increase plant biomass, but no increase in As phytoextraction occurs with the consortium and even a reduction is seen with PVr_9 alone. Conversely, inoculations with PVr_5 and PVr_15 have the capacity of increasing As phytoextraction.
这项温室研究评估了用根际细菌富集土壤对生长在天然富砷土壤上的[植物名称未给出]生长和砷积累的影响。用对100 mM砷酸盐具有抗性的六种细菌组成的联合体进行接种，并将结果与在灭菌土壤上获得的结果进行比较。还分别使用了联合体中挑选出的细菌：与产生吲哚-3-乙酸（IAA）和铁载体并具有ACC脱氨酶活性的[细菌名称未给出]同源的PVr_9，与含有砷酸盐还原酶基因的[细菌名称未给出]同源的PVr_15，以及具有PVr_9和PVr_15所有特征的与[细菌名称未给出]同源的PVr_5。仅在未灭菌土壤上接种联合体的蕨类植物中，叶和根生物量显著增加。单独使用PVr_9时增加幅度更大，而接种PVr_5或PVr_15的植株仅根长度增加。仅接种PVr_9的蕨类植物中砷含量显著降低，而接种PVr_5和PVr_15的植株中砷含量增加。总之，接种联合体和单独接种PVr_9可增加植物生物量，但联合体接种并未增加砷的植物提取量，单独接种PVr_9甚至使砷含量降低。相反，接种PVr_5和PVr_15具有增加砷植物提取量的能力。
