大鼠胎盘和胎膜中前列腺素 Fα 和 E：正常妊娠期间其合成酶的全面免疫组织化学和体内组织水平。
Prostaglandins Fα and E in rat placenta and fetal membrane: a comprehensive immunohistochemistry of their synthetic enzymes and in vivo tissue levels during normal pregnancy.
机构信息
Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
Laboratory of Veterinary Physiology, School of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan.
出版信息
J Vet Med Sci. 2021 Sep 15;83(9):1443-1447. doi: 10.1292/jvms.21-0183. Epub 2021 Jul 31.
We determined a comprehensive immunohistochemistry of putative isoforms of enzymes for prostaglandin (PG) Fα and PGE biosynthesis and these PGs levels in placenta and fetal membrane of normal pregnant rats in vivo. Placenta and fetal membrane showed positive immunoreactions for phospholipase A group 4A, but not group 2A, and cyclooxygenase (COX)-1 rather than COX-2. They showed positive immunoreactions for at least one isoform of each of PGF synthase and PGE synthase with tissue-dependent variations. PGFα and PGE levels in both tissues were highest on day 12 and declined and remained low thereafter. Obtained data would be the basic information on the primary PGs synthesis in rat placenta and fetal membrane in normal pregnancy.
我们在正常妊娠的大鼠体内确定了前列腺素（PG）Fα 和 PGE 生物合成的假定同工型的全面免疫组织化学以及这些 PG 的水平。胎盘和胎膜对磷脂酶 A 组 4A 呈阳性免疫反应，但对组 2A 呈阴性，对环氧化酶（COX）-1 呈阳性，而不是 COX-2。它们对至少一种 PGF 合酶和 PGE 合酶的同工型呈阳性免疫反应，具有组织依赖性变化。这两种组织中的 PGFα 和 PGE 水平在第 12 天最高，然后下降并保持低水平。获得的数据将是大鼠正常妊娠胎盘和胎膜中初级 PG 合成的基础信息。
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