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菠萝蛋白酶和木瓜蛋白酶对复合树脂与牙釉质剪切粘结强度影响的评估。

Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel.

作者信息

Sharafeddin Farahnaz, Yazdanpanah Mohammad Hossein, Jowkar Zahra

机构信息

Department of Operative Dentistry and Biomaterials Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

Oral and Dental Disease Research Center, Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

Int J Dent. 2021 Jul 12;2021:3233639. doi: 10.1155/2021/3233639. eCollection 2021.

Abstract

AIM

This study aimed to evaluate the effects of 6% bromelain and 10% papain enzymes on shear bond strength (SBS) of composite resin to enamel compared to conventional 37% phosphoric acid etching.

MATERIALS AND METHODS

50 human maxillary premolar teeth were randomly divided into 5 groups (G1-G5/ = 10). In G1 and G2, after etching enamel with 37% phosphoric acid for 15 seconds and washing the surface, 10% papain and 6% bromelain enzymes were used, respectively. In G3 and G4, 6% bromelain or 10% papain enzymes were applied on enamel. In G5, the enamel surface was etched with 37% phosphoric acid for 15 seconds. A two-step etch-and-rinse adhesive system (Adper Single Bond 2) was applied. A nanohybrid composite (Z350) was placed using Teflon molds. All the samples were then subjected to the SBS test using a universal testing machine. Data analysis was performed using a one-way ANOVA test followed by the Tukey test. values less than 0.05 were considered significant.

RESULTS

Comparison of the mean SBS between G1, G2, and G5 shows no significant differences ( > 0.05); however, they had higher mean SBS compared with G3 and G4 ( < 0.0001).

CONCLUSIONS

The shear bond strength of composite to enamel was not affected significantly using either 6% bromelain or 10% papain enzymes after 37% phosphoric acid application. Moreover, 6% bromelain and 10% papain enzymes were not as effective as 37% phosphoric acid alone.

摘要

目的

本研究旨在评估6%菠萝蛋白酶和10%木瓜蛋白酶与传统37%磷酸酸蚀相比,对复合树脂与牙釉质之间剪切粘结强度(SBS)的影响。

材料与方法

50颗人类上颌前磨牙随机分为5组(G1 - G5,每组10颗)。在G1和G2组中,用37%磷酸酸蚀牙釉质15秒并冲洗表面后,分别使用10%木瓜蛋白酶和6%菠萝蛋白酶。在G3和G4组中,将6%菠萝蛋白酶或10%木瓜蛋白酶应用于牙釉质。在G5组中,牙釉质表面用37%磷酸酸蚀15秒。应用两步酸蚀冲洗粘结系统(Adper Single Bond 2)。使用聚四氟乙烯模具放置纳米混合复合树脂(Z350)。然后使用万能试验机对所有样本进行SBS测试。使用单因素方差分析测试,随后进行Tukey检验进行数据分析。p值小于0.05被认为具有统计学意义。

结果

G1、G2和G5组之间平均SBS的比较显示无显著差异(p > 0.05);然而,与G3和G4组相比,它们具有更高的平均SBS(p < 0.0001)。

结论

在应用37%磷酸后,使用6%菠萝蛋白酶或10%木瓜蛋白酶对复合树脂与牙釉质之间的剪切粘结强度没有显著影响。此外,6%菠萝蛋白酶和10%木瓜蛋白酶单独使用不如37%磷酸有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55df/8289576/4ad33bc93f08/IJD2021-3233639.001.jpg

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