穿心莲内酯对间变性甲状腺癌的抗肿瘤作用及糖酵解的研究
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.
作者信息
Yang Ke, Wu Ke, Feng Jianguo, Yutian Ling, Zhu Xin, Xu Dong
机构信息
The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
Key Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Hangzhou, Zhejiang 310022, China.
出版信息
Evid Based Complement Alternat Med. 2021 Jul 14;2021:5526581. doi: 10.1155/2021/5526581. eCollection 2021.
OBJECTIVE
To investigate the antitumor effect of andrographolide on the ATC cell lines 8505C and CAL62 and to explore the possible mechanism of the effect.
METHODS
CCK8 and colony formation assays were performed to detect proliferation. Cell migration was tested by scratch assay. Annexin V/PI staining was used to detect cell apoptosis and cell cycle. Glucose and lactic acid kits were carried out to evaluate the glycolysis level after andrographolide treatment. Western blot was used to detect the changes in the apoptosis-related proteins and glycolysis-related enzymes in both 8505C and CAL62 cells.
RESULTS
Treatment with 60 M andrographolide had significant effects on 8505C and CAL62, including inhibition of proliferation, inhibition of migration, arrest of the cell cycle, promotion of apoptosis, and inhibition of glycolysis.
CONCLUSION
Andrographolide has an antitumor effect and can significantly affect glycolysis in ATC cells.
目的
研究穿心莲内酯对人未分化甲状腺癌（ATC）细胞系8505C和CAL62的抗肿瘤作用，并探讨其作用的可能机制。
方法
采用CCK8法和集落形成实验检测细胞增殖。划痕实验检测细胞迁移。Annexin V/PI染色检测细胞凋亡和细胞周期。采用葡萄糖和乳酸试剂盒评估穿心莲内酯处理后细胞的糖酵解水平。蛋白质免疫印迹法检测8505C和CAL62细胞中凋亡相关蛋白和糖酵解相关酶的变化。
结果
60μM穿心莲内酯处理对8505C和CAL62细胞有显著影响，包括抑制增殖、抑制迁移、使细胞周期停滞、促进凋亡以及抑制糖酵解。
结论
穿心莲内酯具有抗肿瘤作用，且能显著影响ATC细胞的糖酵解。
Zotero 插件