Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo 11651, Egypt.
Molecules. 2020 Mar 30;25(7):1582. doi: 10.3390/molecules25071582.
Among the hundreds of reported species, (Labill.) DC. is one of the six that grow in Jordan. Many species of this genus are used in folk medicine to treat a variety of ailments and several biological and pharmacological activities have been ascribed to their essential oil (EO). For this study, the EO obtained from a specimen of grown in Jordan was analyzed by GC-MS. Ninety-six compounds were detected, of which oxygenated monoterpenes was the predominant class (47.9%), followed by non-terpene derivatives (27.9%), while sesquiterpenes represented 14.2% of the total composition. The most abundant compound in the EO was 1,8-cineole (21.7%). The cytotoxic activity of the EO was evaluated against three cancer cell lines (MCF7, A2780 and HT29), and one normal fibroblast cell line (MRC5) by MTT assay. Significant growth inhibition was observed in EO-exposed A2780 and HT29 cells (IC = 12.99 and 14.02 μg/mL, respectively), while MCF7 and MRC5 were less susceptible. The EO induced apoptosis and increased the preG1 events in A2780 cells. 1,8-Cineole, the major constituent of the EO, exhibited submicromolar cytotoxicity against A2780 cells, and was 42 times more selective against MRC5 cells. Its cytotoxicity against A2780 cells was comparable with that of doxorubicin, but 1,8-cineole was more selective for MRC5 normal cells. Interestingly, 1,8-cineole enhanced apoptosis in A2780, and caused a remarkable dose-dependent increase in preG1 events. Thus, 1,8-cineole has demonstrated promising cytotoxic and proapoptotic properties.
在已报道的数百个物种中，（Labill.）DC. 是生长在约旦的六个物种之一。该属的许多物种被民间医学用于治疗各种疾病，并且已经赋予了它们的精油 (EO) 几种生物和药理学活性。在这项研究中，分析了从约旦生长的标本中获得的精油，通过 GC-MS。检测到 96 种化合物，其中含氧单萜是主要类别（47.9%），其次是非萜烯衍生物（27.9%），而倍半萜占总组成的 14.2%。精油中最丰富的化合物是 1,8-桉叶油醇（21.7%）。通过 MTT 测定法评估了精油对三种癌细胞系（MCF7、A2780 和 HT29）和一种正常成纤维细胞系（MRC5）的细胞毒性活性。在暴露于 EO 的 A2780 和 HT29 细胞中观察到显著的生长抑制（IC = 12.99 和 14.02 μg/mL），而 MCF7 和 MRC5 则不易受影响。精油诱导 A2780 细胞凋亡并增加预 G1 事件。精油的主要成分 1,8-桉叶油醇对 A2780 细胞表现出亚微摩尔细胞毒性，对 MRC5 细胞的选择性是 42 倍。它对 A2780 细胞的细胞毒性与阿霉素相当，但 1,8-桉叶油醇对 MRC5 正常细胞的选择性更高。有趣的是，1,8-桉叶油醇增强了 A2780 细胞的凋亡，并导致预 G1 事件的显著剂量依赖性增加。因此，1,8-桉叶油醇表现出有前途的细胞毒性和促凋亡特性。
