Kazmierak Weronika, Korolczuk Agnieszka, Kurzepa Jacek, Czechowska Grażyna, Boguszewska-Czubara Anna, Madro Agnieszka
Department of Gastroenterology with Endoscopic Unit, Medical University of Lublin, Lublin, Poland.
Department of Clinical Pathomorphology, Medical University of Lublin, Lublin, Poland.
Arch Med Sci. 2020 Oct 12;17(4):1100-1108. doi: 10.5114/aoms.2020.99800. eCollection 2021.
Chronic pancreatitis (CP) is a continuing, inflammatory process of the pancreas, characterised by irreversible morphological changes. The identification of pancreatic stellate cells resulted in the development of research on the pathogenesis of CP. Erythropoietin (Epo) regulates the interaction between apoptosis and inflammation of the brain, kidney, and heart muscle. Erythropoietin receptors were also found in the pancreas, in particular on the islet cells. Our objective was to evaluate the influence of Epo on fibrosis and apoptosis in experimental CP.
The experiments were performed on 48 male Wistar rats (250-350 g). The animals were divided into six equal groups (I - control, II - chronic cerulein - induced pancreatitis, III - 1 ml of Epo , IV - 0.5 ml of Epo , V - CP treated with 1 ml Epo, VI - CP treated with 0.5 ml Epo). The blood for gelatinases and pancreata for the morphological examinations and immunohistochemistry were collected.
A slight reduction of interstitial oedema and less severe fibrosis were noticed in the groups treated with Epo. Reduced expression of caspase-3 and α-actin, and a lack of Bcl-2 expression were observed in areas with inflammation. There was no expression of caspase-9 observed in all groups. There were no statistically significant differences between the groups in the activity of gelatinases.
Erythropoietin seems to have the effect of reducing fibrosis and apoptosis in an experimental model of CP.
慢性胰腺炎（CP）是胰腺的一种持续的炎症过程，其特征为不可逆的形态学改变。胰腺星状细胞的发现推动了对CP发病机制的研究。促红细胞生成素（Epo）调节脑、肾和心肌细胞凋亡与炎症之间的相互作用。在胰腺中也发现了促红细胞生成素受体，特别是在胰岛细胞上。我们的目的是评估Epo对实验性CP中纤维化和细胞凋亡的影响。
实验选用48只雄性Wistar大鼠（体重250 - 350克）。动物被分为六个相等的组（I - 对照组，II - 慢性蛙皮素诱导的胰腺炎组，III - 1毫升Epo组，IV - 0.5毫升Epo组，V - 用1毫升Epo治疗的CP组，VI - 用0.5毫升Epo治疗的CP组）。采集用于检测明胶酶的血液以及用于形态学检查和免疫组织化学检测的胰腺组织。
在接受Epo治疗的组中，观察到间质水肿略有减轻，纤维化程度较轻。在炎症区域观察到caspase - 3和α - 肌动蛋白表达降低，且未观察到Bcl - 2表达。所有组均未观察到caspase - 9表达。各组之间明胶酶活性无统计学显著差异。
在CP实验模型中，促红细胞生成素似乎具有减少纤维化和细胞凋亡的作用。
