Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Nanchang University, Nanchang, Jiangxi 330006, China.
Queen Mary University of London, Nanchang University, Nanchang, Jiangxi 330006, China.
J Healthc Eng. 2021 Jul 15;2021:6394514. doi: 10.1155/2021/6394514. eCollection 2021.
Triple negative breast cancer (TNBC) has significantly threatened human health. Many aspects of TNBC are closely related to Wnt/-catenin pathway, and cell apoptosis induced by endoplasmic reticulum stress (ER stress) in TNBC may act as a potential target of non-chemotherapy treatment. However, how ER stress interacts with this pathway in TNBC has not yet been understood. Here, the tunicamycin and LiCl have been applied to MDA-MB-231. The related proteins' expression was measured by western blotting. Moreover, acridine orange/ethidium bromide (AO/EB) staining was applied to test the apoptosis degree of the cells, and cell viability was tested by MTT experiment. Then, we found the ER stress and apoptosis degree of MDA-MB-231 were induced after treatment with tunicamycin. Besides, tunicamycin dose dependently inhibited both Wnt/-catenin pathway and cells viability. Licl, an activator of Wnt/-catenin signaling pathway, could significantly inhibit cell apoptosis. In conclusion, our study found that the activation of ER stress could promote the MDA-MB-231 apoptosis by repressing Wnt/-catenin pathway, which provides some promising prospects and basic mechanism to the further research.
三阴性乳腺癌（TNBC）严重威胁人类健康。TNBC 的许多方面都与 Wnt/-catenin 通路密切相关，内质网应激（ER 应激）诱导的 TNBC 细胞凋亡可能成为非化疗治疗的潜在靶点。然而，ER 应激与该通路在 TNBC 中的相互作用尚不清楚。本研究采用衣霉素和氯化锂（LiCl）处理 MDA-MB-231，通过 Western blot 检测相关蛋白的表达。此外，通过吖啶橙/溴化乙锭（AO/EB）染色检测细胞凋亡程度，通过 MTT 实验检测细胞活力。结果发现，衣霉素处理后 MDA-MB-231 的 ER 应激和凋亡程度增加，且衣霉素呈剂量依赖性抑制 Wnt/-catenin 通路和细胞活力。Wnt/-catenin 信号通路的激活剂 LiCl 可显著抑制细胞凋亡。综上，本研究发现 ER 应激的激活可通过抑制 Wnt/-catenin 通路促进 MDA-MB-231 细胞凋亡，为进一步研究提供了有前景的前景和基础机制。
