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hsa_circ_0005505在人类颅内动脉瘤破裂中的潜在作用

The Potential Role of hsa_circ_0005505 in the Rupture of Human Intracranial Aneurysm.

作者信息

Chen Xin, Yang Shuzhe, Yang Junhua, Liu Qingyuan, Li Maogui, Wu Jun, Wang Hao, Wang Shuo

机构信息

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

China National Clinical Research Center for Neurological Diseases, Beijing, China.

出版信息

Front Mol Biosci. 2021 Jul 14;8:670691. doi: 10.3389/fmolb.2021.670691. eCollection 2021.

DOI:10.3389/fmolb.2021.670691
PMID:34336924
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8316638/
Abstract

Recently, abundant number of studies have revealed many functions of circular RNAs in multiple diseases, however, the role of circular RNA in the rupture of human intracranial aneurysm is still unknown. This study aims to explore the potential functions of circular RNA in the rupture of human intracranial aneurysms. The differentially expressed circular RNAs between un-ruptured intracranial aneurysms ( = 5) and ruptured intracranial aneurysms ( = 5) were analyzed with the Arraystar human circRNAs microarray. Quantitative real-time PCR (qPCR) was used to verify the results of the circRNA microarray. The role of circular RNA in intracranial aneurysm rupture was assessed . MTT assay, CCK-8 assay, Caspase3/7 assay, assay of cell apoptosis and Celigo wound healing was conducted to evaluate the relationship between circular RNA and the rupture of human intracranial aneurysms. A total of 13,175 circRNA genes were detected. Among them 63 circRNAs upregulated and 54 circRNAs downregulated significantly in ruptured intracranial aneurysms compared with un-ruptured intracranial aneurysms ( < 0.05 Fold Change > 1.5). Five upregulated circRNAs were selected for further study (hsa_circ_0001947, hsa_circ_0043001, hsa_circ_0064557, hsa_circ_0058514, hsa_circ_0005505). The results of qPCR showed only hsa_circ_0005505 significantly upregulated ( < 0.05). The expression of hsa_circ_0005505 was higher in ruptured intracranial aneurysm tissues. And our data showed that hsa_circRNA_005505 promotes the proliferation, migration and suppresses the apoptosis of vascular smooth muscle cell. This study revealed an important role of hsa_circ_0005505 in the proliferation, migration and apoptosis of vascular smooth muscle cell, and indicated that hsa_circ_0005505 may associate with the pathological process of intracranial aneurysms.

摘要

最近，大量研究揭示了环状RNA在多种疾病中的许多功能，然而，环状RNA在人类颅内动脉瘤破裂中的作用仍然未知。本研究旨在探讨环状RNA在人类颅内动脉瘤破裂中的潜在功能。使用Arraystar人类环状RNA微阵列分析未破裂颅内动脉瘤（n = 5）和破裂颅内动脉瘤（n = 5）之间差异表达的环状RNA。采用定量实时PCR（qPCR）验证环状RNA微阵列的结果。评估环状RNA在颅内动脉瘤破裂中的作用。进行MTT法、CCK-8法、Caspase3/7法、细胞凋亡检测和Celigo伤口愈合检测，以评估环状RNA与人类颅内动脉瘤破裂之间的关系。共检测到13175个环状RNA基因。其中，与未破裂颅内动脉瘤相比，破裂颅内动脉瘤中有63个环状RNA显著上调，54个环状RNA显著下调（P < 0.05，倍数变化> 1.5）。选择5个上调的环状RNA进行进一步研究（hsa_circ_0001947、hsa_circ_0043001、hsa_circ_0064557、hsa_circ_0058514、hsa_circ_0005505）。qPCR结果显示只有hsa_circ_0005505显著上调（P < 0.05）。hsa_circ_0005505在破裂颅内动脉瘤组织中的表达较高。并且我们的数据表明hsa_circRNA_005505促进血管平滑肌细胞的增殖、迁移并抑制其凋亡。本研究揭示了hsa_circ_0005505在血管平滑肌细胞增殖、迁移和凋亡中的重要作用，并表明hsa_circ_0005505可能与颅内动脉瘤的病理过程相关。

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