聚合酶链反应作为牙周病学中杰出的分子诊断技术的观点。
A View on Polymerase Chain Reaction as an Outstanding Molecular Diagnostic Technique in Periodontology.
机构信息
Department of Periodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Biomed Res Int. 2021 Jul 19;2021:9979948. doi: 10.1155/2021/9979948. eCollection 2021.
OBJECTIVES
This study presents a discussion on the fundamentals of polymerase chain reaction (PCR) and its use as a diagnostic tool in periodontology.
MATERIALS AND METHODS
A computer-aided as well as hand-made search in PubMed and Scopus indexed journals (relevant to the topic) was done by keywords of molecular technique in periodontology, PCR, applications of PCR, and PCR in periodontics. Only the papers in the English language and outlining PCR and its association with periodontology were collected and utilized to provide a succinct review. There was no limitation for publication time.
RESULTS
The results of our search showed that PCR has turned into a standard in diagnosis in the field of periodontology. A variety of researches has demonstrated that its sensitive, and specific characteristics make it a quick and effective technique of recognition, identification, and quantification of microorganisms. Identification of various immunoinflammatory markers at the mRNA expression level as well as ascertaining gene-related polymorphisms can also be performed.
CONCLUSIONS
The mechanisms of periodontal disease can further become clarified using PCR. . PCR as a diagnostic method can play a main part in the validation of the clinical diagnosis of periodontal disease indicating the reason, pathogenesis, clinical steps, progress, and prognosis of the disease.
目的
本研究讨论了聚合酶链反应（PCR）的基本原理及其在牙周病学中的诊断工具应用。
材料与方法
通过牙周病学中的分子技术、PCR、PCR 的应用和 PCR 在牙周病学中的关键词，在 PubMed 和 Scopus 索引期刊（与主题相关）中进行了计算机辅助和手工搜索。仅收集和利用了概述 PCR 及其与牙周病学关联的英文论文，以提供简洁的综述。对出版时间没有限制。
结果
我们搜索的结果表明，PCR 已成为牙周病学诊断领域的标准。许多研究表明，其敏感和特异性使其成为一种快速有效的识别、鉴定和定量微生物的技术。还可以进行各种免疫炎症标志物在 mRNA 表达水平的鉴定以及确定与基因相关的多态性。
结论
PCR 可以进一步阐明牙周病的发病机制。PCR 作为一种诊断方法，可以在验证牙周病的临床诊断中发挥主要作用，指出疾病的原因、发病机制、临床步骤、进展和预后。
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