HEK293T 和 C2C12 细胞的细胞准备和基因转导方案。
Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells.
机构信息
State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China.
Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
出版信息
STAR Protoc. 2021 Jul 17;2(3):100497. doi: 10.1016/j.xpro.2021.100497. eCollection 2021 Sep 17.
Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression using as an example. This protocol enables sufficient and efficient gene expression for the downstream functional analysis. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).
目的基因在一般和特定细胞类型中的过表达对于基因功能的机制研究很重要。这里，我们提供了一个使用 as an example 进行细胞培养、HEK293T 细胞中转染质粒和 C2C12 细胞中腺病毒感染以过表达基因的分步方案。该方案可实现下游功能分析所需的充足且高效的基因表达。有关该方案使用和执行的完整详细信息，请参考 Jiang 等人（2021 年）。
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