在培养细胞的胞质分裂过程中测量拉普拉斯压力和成像肌动蛋白皮层。
Measuring Laplace pressure and imaging the actin cortex during cytokinesis in cultured cells.
机构信息
State Key Laboratory of Nonlinear Mechanics and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China.
School of Engineering Science, University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
STAR Protoc. 2022 Mar 15;3(2):101239. doi: 10.1016/j.xpro.2022.101239. eCollection 2022 Jun 17.
Laplace pressure is an important regulator of cell dynamics and behavior during cytokinesis. Here, we provide a protocol to measure Laplace pressure in cultured cells using a micropipette and describe the steps for imaging the actin cortex during cytokinesis. The quantification steps allow tracing dynamic change in Laplace pressure and displaying dynamic response of the actin cortex during cytokinesis in HeLa cells. This protocol can be applied to any cultured cell type during various stages of cell division. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
拉普拉斯压力是细胞动力学和胞质分裂过程中细胞行为的重要调节因子。在这里，我们提供了一种使用微管测量培养细胞中拉普拉斯压力的方案，并描述了在胞质分裂过程中对肌动蛋白皮层进行成像的步骤。定量步骤允许追踪拉普拉斯压力的动态变化，并显示 HeLa 细胞胞质分裂过程中肌动蛋白皮层的动态响应。该方案可应用于细胞分裂的各个阶段的任何培养细胞类型。有关此方案的使用和执行的完整详细信息，请参阅 Wang 等人。（2021 年）。
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