Department of Pathology, University of California, San Diego, CA 92093, USA.
Department of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USA.
STAR Protoc. 2021 Jul 17;2(3):100680. doi: 10.1016/j.xpro.2021.100680. eCollection 2021 Sep 17.
Here, we describe the use of polarized patient enteroid-derived monolayers (EDMs) to assess the impact of e-cigarettes on the human gut barrier. These EDMs can be adapted to culture in a 96-well plate for high-throughput screening. We model the effect of e-cigarettes by combining pathogens, enteroids, and e-cigarette vapor-infused media and assess gut barrier integrity, bacterial internalization, and inflammatory response of the gut epithelium. This protocol can be used to assess the effects of e-cigarette components on gut functions. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).
在这里，我们描述了使用极化的患者类器官衍生的单层细胞（EDM）来评估电子烟对人类肠道屏障的影响。这些 EDM 可以适应在 96 孔板中进行高通量筛选的培养。我们通过组合病原体、类器官和电子烟蒸汽注入的培养基来模拟电子烟的效果，并评估肠道屏障的完整性、细菌内化和肠道上皮的炎症反应。本方案可用于评估电子烟成分对肠道功能的影响。有关该方案使用和执行的完整详细信息，请参阅 Sharma 等人。（2021 年）。
