Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Department of Orthopaedic Surgery, Stanford University, Stanford, CA 94305, USA.
Cell Rep Med. 2021 Jul 21;2(7):100343. doi: 10.1016/j.xcrm.2021.100343. eCollection 2021 Jul 20.
Regenerative medicine approaches utilizing stem cells offer a promising strategy to address tendinopathy, a class of common tendon disorders associated with pain and impaired function. Tendon progenitor cells (TPCs) are important in healing and maintaining tendon tissues. Here we provide a comprehensive single cell transcriptomic profiling of TPCs from three normal and three clinically classified tendinopathy samples in response to mechanical stimuli. Analysis reveals seven distinct TPC subpopulations including subsets that are responsive to the mechanical stress, highly clonogenic, and specialized in cytokine or growth factor expression. The single cell transcriptomic profiling of TPCs and their subsets serves as a foundation for further investigation into the pathology and molecular hallmarks of tendinopathy in mechanical stimulation conditions.
利用干细胞的再生医学方法为治疗腱病提供了一种很有前途的策略，腱病是一类与疼痛和功能障碍相关的常见肌腱疾病。肌腱祖细胞（TPCs）在愈合和维持肌腱组织中起着重要作用。在这里，我们对来自三个正常和三个临床分类腱病样本的 TPCs 对机械刺激的反应进行了全面的单细胞转录组谱分析。分析揭示了七个不同的 TPC 亚群，包括对机械应激有反应、高克隆形成和专门表达细胞因子或生长因子的亚群。TPC 及其亚群的单细胞转录组谱分析为进一步研究机械刺激条件下腱病的病理学和分子特征奠定了基础。
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