Siu Wing Sum, Ma Hui, Ko Chun Hay, Shiu Hoi Ting, Cheng Wen, Lee Yuk Wa, Kot Cheuk Hin, Leung Ping Chung, Lui Pauline Po Yee
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
Cells. 2023 Sep 6;12(18):2222. doi: 10.3390/cells12182222.
The pathogenesis of plantar fasciitis is unclear, which hampers the development of an effective treatment. The altered fate of plantar fascia stem/progenitor cells (PFSCs) under overuse-induced inflammation might contribute to the pathogenesis. This study aimed to isolate rat PFSCs and compared their stem cell-related properties with bone marrow stromal cells (BMSCs). The effects of inflammation and intensive mechanical loading on PFSCs' functions were also examined. We showed that plantar fascia-derived cells (PFCs) expressed common MSC surface markers and embryonic stemness markers. They expressed lower but higher and , proliferated faster and formed more colonies compared to BMSCs. Although PFCs showed higher chondrogenic differentiation potential, they showed low osteogenic and adipogenic differentiation potential upon induction compared to BMSCs. The expression of ligament markers was higher in PFCs than in BMSCs. The isolated PFCs were hence PFSCs. Both IL-1β and intensive mechanical loading suppressed the mRNA expression of ligament markers but increased the expression of inflammatory cytokines and matrix-degrading enzymes in PFSCs. In summary, rat PFSCs were successfully isolated. They had poor multi-lineage differentiation potential compared to BMSCs. Inflammation after overuse altered the fate and inflammatory status of PFSCs, which might lead to poor ligament differentiation of PFSCs and extracellular matrix degeneration. Rat PFSCs can be used as an in vitro model for studying the effects of intensive mechanical loading-induced inflammation on matrix degeneration and erroneous stem/progenitor cell differentiation in plantar fasciitis.
足底筋膜炎的发病机制尚不清楚，这阻碍了有效治疗方法的开发。过度使用引起的炎症状态下，足底筋膜干/祖细胞（PFSCs）命运的改变可能与发病机制有关。本研究旨在分离大鼠PFSCs，并将其干细胞相关特性与骨髓基质细胞（BMSCs）进行比较。还研究了炎症和高强度机械负荷对PFSCs功能的影响。我们发现，足底筋膜来源的细胞（PFCs）表达常见的间充质干细胞表面标志物和胚胎干性标志物。与BMSCs相比，它们表达较低的 但较高的 和 ，增殖更快，形成的集落更多。尽管PFCs显示出更高的软骨分化潜能，但与BMSCs相比，诱导后它们的成骨和成脂分化潜能较低。PFCs中韧带标志物的表达高于BMSCs。因此，分离出的PFCs即为PFSCs。IL-1β和高强度机械负荷均抑制了PFSCs中韧带标志物的mRNA表达，但增加了炎症细胞因子和基质降解酶的表达。总之，大鼠PFSCs被成功分离。与BMSCs相比，它们的多向分化潜能较差。过度使用后的炎症改变了PFSCs的命运和炎症状态，这可能导致PFSCs韧带分化不良和细胞外基质退变。大鼠PFSCs可作为体外模型，用于研究高强度机械负荷诱导的炎症对足底筋膜炎中基质退变和错误的干/祖细胞分化的影响。
