EICU, the First People's Hospital of Wenling, Wenling, PR China.
First Aid Center, the First People's Hospital of Wenling, Wenling, PR China.
J Biomater Sci Polym Ed. 2021 Oct;32(15):2009-2027. doi: 10.1080/09205063.2021.1953237. Epub 2021 Aug 1.
In the present study, curcumin loaded chitosan/poly ethylene glycol nanomaterial (CUR loaded CH/PEG/AgNPs) was fabricated and characterized for wound healing efficiency after fracture surgery. The interaction of functional groups and crystal nature were recorded under FTIR and XRD spectrometer and reveals that the stabilization and purity of NPs was mediated by OH/NH groups in chitosan. FESEM showed the presence of spherical and well dispersed particles. The average size of the particle was 13.48 nm. The CUR loaded CH/PEG/AgNPs showed higher swelling capacity (495.6 g/g) in phosphate buffer saline compared to water (140.2 g/g). The drug loading efficiency was higher in CUR loaded CH/PEG/AgNPs compared to CH/PEG films as recorded by the absorbance peak at 460 nm corresponds to curcumin in the composite. A dose dependent cytotoxicity of CUR loaded CH/PEG/AgNPs was noticed on Vero cells. The viability of Vero cells was increased to 96.5% at 100 μg/mL. A remarkable change in Vero cells such as condensed nuclei and membrane blabbing was noticed in cells treated with CUR loaded CH/PEG/AgNPs. A greater inhibition of and was noticed at 24 h and 48 h treated with CUR loaded CH/PEG/AgNPs. A greater healing effect by increasing the wound contraction (98% on day 12) was observed with CUR loaded CH/PEG/AgNPs compared to control. Histopathological examination demonstrated that CUR loaded CH/PEG/AgNPs showed complete tissue regeneration in wound excised rats. The results of this study conclude that CUR loaded CH/PEG/AgNPs could be promising candidate to prevent microbial infections in wound, healing wound rapidly and inhibit the proliferation of apoptotic cells. Thus, CUR loaded CH/PEG/AgNPs could be a potential therapeutic agent with broad spectrum applications in the future.HighlightsA new approach was used to develop curcumin-loaded chitosan/poly(ethylene glycol)/AgNPs.The CUR-loaded CH/PEG/AgNPs were confirmed to be crystals by XRD analysis.The prepared CH/PEG/AgNPs were spherical and averaged 13.48 nm in size.The growth of and were inhibited mostly by CH/PEG/AgNPs treatment.CUR loaded CH/PEG/AgNPs showed complete tissue regeneration in wound excised mice.
在本研究中，制备了负载姜黄素的壳聚糖/聚乙二醇纳米材料（CUR 负载 CH/PEG/AgNPs），并对其在骨折手术后的伤口愈合效率进行了评价。通过傅里叶变换红外光谱仪和 X 射线衍射仪记录了功能基团的相互作用和晶体性质，结果表明壳聚糖中的 OH/NH 基团介导了 NPs 的稳定性和纯度。FESEM 显示存在球形且分散良好的颗粒。颗粒的平均粒径为 13.48nm。与水（140.2g/g）相比，CUR 负载 CH/PEG/AgNPs 在磷酸盐缓冲盐水中具有更高的溶胀能力（495.6g/g）。通过在复合材料中对应于姜黄素的 460nm 处的吸光度峰记录到，CUR 负载 CH/PEG/AgNPs 的药物负载效率高于 CH/PEG 薄膜。在 Vero 细胞上，CUR 负载 CH/PEG/AgNPs 表现出剂量依赖性细胞毒性。当 CUR 负载 CH/PEG/AgNPs 的浓度为 100μg/mL 时，Vero 细胞的活力增加到 96.5%。用 CUR 负载 CH/PEG/AgNPs 处理的 Vero 细胞出现明显的变化，如核浓缩和细胞膜起泡。在 24h 和 48h 时，用 CUR 负载 CH/PEG/AgNPs 处理， 和 的抑制作用更大。与对照相比，CUR 负载 CH/PEG/AgNPs 观察到更大的伤口收缩愈合效果（第 12 天增加 98%）。组织病理学检查表明，在切除大鼠的伤口中，CUR 负载 CH/PEG/AgNPs 显示出完全的组织再生。这项研究的结果表明，CUR 负载 CH/PEG/AgNPs 可作为一种有前途的候选药物，用于预防伤口中的微生物感染，快速愈合伤口，并抑制凋亡细胞的增殖。因此，CUR 负载 CH/PEG/AgNPs 可能是一种具有广谱应用前景的潜在治疗药物。
