School of Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju, South Korea; Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju, South Korea; Division of Mechanical Design Engineering, Jeonbuk National University, Jeonju, South Korea.
Int J Biol Macromol. 2024 Apr;263(Pt 1):130217. doi: 10.1016/j.ijbiomac.2024.130217. Epub 2024 Feb 17.
A wound dressing material should inhibit infections that may occur at the wound site, and at the same time, it should enhance the healing process. In this study, we developed an amikacin sulphate (AK) incorporated chitosan (Ch) and Diopside nanoparticles composite dressing (Ch-nDE-AK) for controlling wound infection and healing. The diopside nanoparticles (nDE) were prepared using sol-gel synthesis and characterized using XRD, FT-IR, and FESEM. nDE shows a size range of 142 ± 31 nm through FESEM analysis. Later, the developed composite dressing was characterized using SEM, EDS, and FT-IR analysis. Ch-nDE-AK dressing possesses a porous nature that will aid in easy cell infiltration and proliferation. The swelling studies indicated the expansion capability of the scaffold when applied to the injured site. Ch-nDE-AK scaffold showed a 69.6 ± 8.2 % amikacin sulphate release up to 7 days, which indicates the sustained release of the drug from Ch-nDE-AK scaffold. The drug release data was subjected to various kinetics models and was observed to follow the Higuchi model. The scaffold showed antibacterial activity against ATCC strains of S. aureus and E. coli for 7 days by in vitro. Ch-nDE-AK scaffold also showed antibacterial activity against S. aureus and E. coli clinical strains in vitro. The ex vivo antibacterial study confirmed the antibacterial ability of Ch-nDE-AK scaffold against S. aureus and E. coli. Ch-nDE-AK scaffold also exhibits anti-biofilm activity against S. aureus and E. coli. The Ch-nDE-AK scaffold showed cytocompatibility and cell attachment to fibroblast cells. Additionally, the scratch assay using fibroblast cells confirmed the role of the nDE in the scaffold, helping in cell migration. Thus, the developed Ch-nDE-AK dressing can potentially be used to treat infectious wound healing.
一种伤口敷料材料应能抑制可能发生在伤口部位的感染，同时应能促进愈合过程。在本研究中，我们开发了一种硫酸阿米卡星（AK）掺入壳聚糖（Ch）和透辉石纳米粒子复合敷料（Ch-nDE-AK），用于控制伤口感染和愈合。透辉石纳米粒子（nDE）通过溶胶-凝胶合成制备，并通过 XRD、FT-IR 和 FESEM 进行表征。FESEM 分析表明 nDE 的尺寸范围为 142±31nm。随后，通过 SEM、EDS 和 FT-IR 分析对所开发的复合敷料进行了表征。Ch-nDE-AK 敷料具有多孔性质，这将有助于细胞容易地渗透和增殖。溶胀研究表明，当应用于受伤部位时，支架具有扩展能力。Ch-nDE-AK 支架显示出高达 7 天的硫酸阿米卡星 69.6±8.2%的释放，这表明药物从 Ch-nDE-AK 支架中持续释放。药物释放数据符合各种动力学模型，并观察到符合 Higuchi 模型。支架在体外对 ATCC 金黄色葡萄球菌和大肠杆菌菌株显示出 7 天的抗菌活性。Ch-nDE-AK 支架在体外对金黄色葡萄球菌和大肠杆菌临床株也显示出抗菌活性。体外抗菌研究证实了 Ch-nDE-AK 支架对金黄色葡萄球菌和大肠杆菌的抗菌能力。Ch-nDE-AK 支架还表现出抗金黄色葡萄球菌和大肠杆菌生物膜的活性。Ch-nDE-AK 支架对成纤维细胞表现出细胞相容性和细胞附着。此外，使用成纤维细胞的划痕试验证实了 nDE 在支架中的作用，有助于细胞迁移。因此，所开发的 Ch-nDE-AK 敷料有可能用于治疗感染性伤口愈合。
