Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
School of Life Science, Jilin University, Changchun, Jilin Province, China.
Int Immunopharmacol. 2021 Oct;99:108015. doi: 10.1016/j.intimp.2021.108015. Epub 2021 Jul 31.
Dioscin is a natural steroid saponin derived from plants of the genus Dioscoreaceae. Previous studies have proved its effects of antibacterial, anti-inflammatory and hypolipidemic. In this study, our aim was to explore the protective effect and preliminary mechanism of Dioscin on dextran sulfate sodium (DSS)-induced colitis in mice. The results showed that Dioscin reduced DSS-induced disease activity index (DAI) increase, colon length shortening and colon pathological damage. In addition, Dioscin reduced excessive inflammation by reversing the cytokines levels, reducing intestinal macrophage infiltration and promoting macrophage polarization to M2 phenotype. At the same time, Dioscin maintained the intestinal barrier function by increasing the expression of zonula occludens-1 (ZO-1), occludin and mucin (Muc)-2. Moreover, Dioscin inhibited NF-κB, MAPK signaling and nucleotide oligomerization domain-like receptor family pyrin domain ontaining 3(NLRP3) inflammasome pathway in DSS-induced colitis. These results suggest that Dioscin is a competent candidate for ulcerative colitis (UC) therapy in the future.
薯蓣皂苷元是一种天然甾体皂苷，来源于薯蓣科植物。先前的研究已经证明了它具有抗菌、抗炎和降血脂的作用。在这项研究中，我们的目的是探讨薯蓣皂苷元对葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的保护作用及其初步机制。结果表明，薯蓣皂苷元可降低 DSS 诱导的疾病活动指数（DAI）升高、结肠缩短和结肠病理损伤。此外，薯蓣皂苷元通过逆转细胞因子水平、减少肠道巨噬细胞浸润并促进巨噬细胞向 M2 表型极化来减轻过度炎症。同时，薯蓣皂苷元通过增加紧密连接蛋白-1（ZO-1）、闭合蛋白和黏蛋白（Muc）-2 的表达来维持肠道屏障功能。此外，薯蓣皂苷元抑制了 DSS 诱导的结肠炎中的 NF-κB、MAPK 信号通路和核苷酸结合寡聚结构域样受体家族含pyrin 结构域 3（NLRP3）炎性小体途径。这些结果表明，薯蓣皂苷元是未来溃疡性结肠炎（UC）治疗的一个有潜力的候选药物。
