Lapps W, Hogue B G, Brian D A
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Adv Exp Med Biol. 1987;218:123-9. doi: 10.1007/978-1-4684-1280-2_14.
The nucleotide sequence of the matrix (M) protein gene of the bovine coronavirus (BCV) was determined by sequencing cDNA clones derived from genomic RNA. The gene was found to map at the 5' side of the nucleocapsid protein gene and its sequence predicts a protein of 230 amino acids having a molecular weight of 26,376. The BCV M protein shares extensive sequence homology with the matrix protein of the mouse hepatitis coronavirus (MHV) but differs notably in the amino terminal region external to the virion envelope where BCV apparently uses at least two of its six potential O-glycosylation sites.
通过对源自基因组RNA的cDNA克隆进行测序，确定了牛冠状病毒（BCV）基质（M）蛋白基因的核苷酸序列。发现该基因定位于核衣壳蛋白基因的5'端，其序列预测有一个由230个氨基酸组成、分子量为26376的蛋白质。BCV的M蛋白与小鼠肝炎冠状病毒（MHV）的基质蛋白有广泛的序列同源性，但在病毒粒子包膜外部的氨基末端区域有显著差异，在该区域BCV显然使用了其六个潜在O-糖基化位点中的至少两个。
