Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133, Milan, Italy.
Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche, Piazza Leonardo da Vinci 32, 20133, Milan, Italy.
Sci Rep. 2021 Aug 3;11(1):15723. doi: 10.1038/s41598-021-95266-2.
In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly to the formation of inherently aligned tomographies. Our method generates sharp reconstructions, with the same accuracy reachable after sub-pixel alignment but with improved point-spread-function. The procedure can be performed simultaneously with deconvolution further increasing the reconstruction resolution.
在多视角荧光显微镜中，需要仔细对齐每个角度采集，以获得最佳的体积重建。在这里，我们提出了一种基于自相关反演的简洁方案，可直接生成固有对齐的层析成像。我们的方法生成的重建结果非常清晰，在亚像素对齐后可以达到相同的精度，但点扩散函数得到了改善。该过程可以与去卷积同时进行，进一步提高重建分辨率。
