Department of Nephrology, China-Japan Union Hospital of Jilin University Changchun, Jilin, China.
Arch Physiol Biochem. 2024 Feb;130(1):29-37. doi: 10.1080/13813455.2021.1959617. Epub 2021 Aug 4.
This study sought to examine the anti-diabetic effect of nobiletin on streptozotocin (STZ)/cadmium (Cd)-induced diabetic nephrotoxic (DN) rats. The DN was induced using STZ (40 mg/kg b.w) intraperitoneally and Cd through drinking water for 12 weeks. The DN rats were treated with nobiletin of different concentrations (10, 20, and 40 mg/kg/BW). The STZ/Cd-induced DN leads to a significantly increased of the glucose levels, glycosylated haemoglobin, hepatic and kidney function markers, lipid peroxidation levels, and reduction of insulin levels, total haemoglobin, body weight, and antioxidant status markers. Our finding that nobiletin pathological impairment and diminished infiltration of neutrophil in kidney tubules and all biochemical enzymes were near normal levels in DN. More essentially, nobiletin strongly impedes the protein expression of renal nuclear NF-κB p65. Bax protein expression was significantly downregulated and elevated protein expression Bcl-2 was recorded in DN rats. These results show that nobiletin possesses antioxidant as well as anti-diabetic activities and thereby reduces chronic kidney diseases in rats.
本研究旨在探讨川陈皮素对链脲佐菌素（STZ）/镉（Cd）诱导的糖尿病肾病（DN）大鼠的抗糖尿病作用。DN 是通过腹腔内注射 STZ（40mg/kg b.w）和饮用水中 12 周 Cd 诱导的。DN 大鼠用不同浓度（10、20 和 40mg/kg/BW）的川陈皮素进行治疗。STZ/Cd 诱导的 DN 导致血糖水平、糖化血红蛋白、肝肾功能标志物、脂质过氧化水平显著升高，胰岛素水平、总血红蛋白、体重和抗氧化状态标志物降低。我们发现，川陈皮素对肾脏小管的病理损伤和中性粒细胞浸润有明显的改善作用，所有生化酶均接近 DN 的正常水平。更重要的是，川陈皮素能强烈抑制核 NF-κB p65 的蛋白表达。DN 大鼠 Bax 蛋白表达显著下调，Bcl-2 蛋白表达升高。这些结果表明，川陈皮素具有抗氧化和抗糖尿病作用，从而减少了大鼠的慢性肾脏病。
