CNRS, Sorbonne Université, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, 29680 Roscoff, Brittany, France.
University of Liège, Gembloux Agro-Bio Tech, Microbiology and Genomic Laboratory, TERRA building, avenue de la Faculté 2b, 5030 Gembloux, Belgium.
Int J Syst Evol Microbiol. 2021 Aug;71(8). doi: 10.1099/ijsem.0.004913.
Four marine bacterial strains were isolated from a thallus of the brown alga collected in Roscoff, France. Cells were Gram-stain-negative, strictly aerobic, non-flagellated, gliding, rod-shaped and grew optimally at 25-30 °C, at pH 7-8 and with 2-4 % NaCl. Phylogenetic analyses of their 16S rRNA gene sequences showed that the bacteria were affiliated to the genus (family , phylum ). The four strains exhibited 97.8-100 % 16S rRNA gene sequence similarity values among themselves, 97.9-99.1 % to the type strains of KMM 3526 and KMM 3676, and less than 99 % to other species of the genus . The DNA G+C content of the four strains ranged from 36.7 to 37.7 mol%. Average nucleotide identity and digital DNA-DNA hybridization calculations between the new strains and other members of the genus resulted in values of 76.4-88.9 % and below 38.5 %, respectively. Phenotypic, phylogenetic and genomic analyses showed that the four strains are distinct from species of the genus with validly published names. They represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed with Asnod1-F08 (RCC6906=KMM 6823=CIP 111902) and Asnod2-B07-B (RCC6908=KMM 6825=CIP 111904), respectively, as the type strains.
从法国罗什福尔采集的褐藻藻体中分离到 4 株海洋细菌。细胞革兰氏染色阴性,严格需氧,无鞭毛,滑行,杆状,最佳生长温度为 25-30°C,pH7-8,盐度 2-4%。16S rRNA 基因序列的系统发育分析表明,这些细菌属于(科,门,纲)。这四个菌株之间的 16S rRNA 基因序列相似度为 97.8-100%,与 KMM 3526 和 KMM 3676 的模式菌株相似度为 97.9-99.1%,与属内其他种的相似度小于 99%。这四个菌株的 DNA G+C 含量范围为 36.7-37.7mol%。新菌株与属内其他成员之间的平均核苷酸同一性和数字 DNA-DNA 杂交计算值分别为 76.4-88.9%和低于 38.5%。表型、系统发育和基因组分析表明,这四个菌株与已发表的有效名称的属内种明显不同。它们代表了属内的两个新种,建议分别将 Asnod1-F08(RCC6906=KMM 6823=CIP 111902)和 Asnod2-B07-B(RCC6908=KMM 6825=CIP 111904)作为这两个新种的模式菌株。