Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6240-6250. doi: 10.1099/ijsem.0.004522.
Three Gram-stain-negative, non-motile, rod-shaped strains, designated 72, NH166 and 40DY170, were isolated from seawater samples of the West Pacific Ocean, South China Sea and West Pacific Ocean, respectively. The 16S rRNA gene sequence similarity results revealed that strains 72and NH166 were most closely related to Ar-22, JCM 17757, KCTC 23501, KCTC 22173 and MT-229 with 97.2-98.0% sequence similarity. 16S rRNA gene sequence analysis also indicated that strain 40DY170 was most closely related to DSM 13258, JCM 11811, KCTC 22173 and 501str8 with 97.6-98.1% sequence similarity. The 16S rRNA gene sequence similarity values among strains 72, NH166 and 40DY170 were 96.5-99.2%. Phylogenetic analyses indicated that three new isolates represented three novel species by forming two distinctive lineages within the genus . The DNA G+C contents of strain 72, NH166 and 40DY170 were 43.4, 43.4 and 42.4 mol%, respectively. The average nucleotide identity and DNA-DNA hybridization values between strains 72, NH166, 40DY170 and the reference strains were 76.5-93.5% and 19.2-53.5%, respectively. The sole respiratory quinone in all strains was menaquinone-6. Their major fatty acids were iso-C 3-OH, iso-C and iso-C G. The major polar lipids of strains 72 and NH166 were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The major polar lipids of strain 40DY170 were phosphatidylglycerol, one unidentified phospholipid, one unidentified aminolipid and two unidentified lipids. On the basis of their distinct taxonomic characteristics, the three isolates represent three novel species of the genus , for which the names sp. nov. (type strain 72=KCTC 62229=MCCC 1K03350), sp. nov. (NH166=KCTC 62228=MCCC 1K03449) and sp. nov. (40DY170=KCTC 72200=MCCC 1K03569) are proposed.
三株革兰氏阴性、非运动、杆状的菌株，分别命名为 72 株、NH166 株和 40DY170 株，分别从西太平洋、南海和西太平洋的海水样本中分离得到。16S rRNA 基因序列相似性结果表明，菌株 72 和 NH166 与 Ar-22、JCM 17757、KCTC 23501、KCTC 22173 和 MT-229 的序列相似性最高，为 97.2-98.0%。16S rRNA 基因序列分析还表明，菌株 40DY170 与 DSM 13258、JCM 11811、KCTC 22173 和 501str8 的序列相似性最高，为 97.6-98.1%。72 株、NH166 株和 40DY170 株之间的 16S rRNA 基因序列相似性为 96.5-99.2%。系统发育分析表明，三个新分离株在属内形成两个不同的分支，代表三个新种。菌株 72、NH166 和 40DY170 的 DNA G+C 含量分别为 43.4、43.4 和 42.4mol%。菌株 72、NH166、40DY170 与参考菌株之间的平均核苷酸同一性和 DNA-DNA 杂交值分别为 76.5-93.5%和 19.2-53.5%。所有菌株的唯一呼吸醌均为menaquinone-6。它们的主要脂肪酸是 iso-C 3-OH、iso-C 和 iso-C G。菌株 72 和 NH166 的主要极性脂质是磷脂酰乙醇胺、一种未鉴定的氨基脂和两种未鉴定的脂质。菌株 40DY170 的主要极性脂质是磷脂酰甘油、一种未鉴定的磷脂、一种未鉴定的氨基脂和两种未鉴定的脂质。基于其独特的分类特征，这三个分离株代表了属的三个新种，分别命名为 sp. nov.（模式菌株 72=KCTC 62229=MCCC 1K03350）、sp. nov.（NH166=KCTC 62228=MCCC 1K03449）和 sp. nov.（40DY170=KCTC 72200=MCCC 1K03569）。
