Department of Food Science, College of Food and Agriculture, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Department of Biological Sciences, University of Limerick, Limerick, Ireland.
Food Chem. 2022 Jan 15;367:130661. doi: 10.1016/j.foodchem.2021.130661. Epub 2021 Jul 22.
Cow (CwC) and camel casein (CaC) hydrolysates were generated using Alcalase™ (CwCA and CaCA) and Pronase-E (CwCP and CaCP) each for 3 and 6 h, and investigated for their potential to inhibit key lipid digesting enzymes i.e., pancreatic lipase (PL) and cholesteryl esterase (CE). Results revealed stronger PL and CE inhibition by CaC hydrolysates compared to CwC. Potent hydrolysates (CwCP-3 h and CaCA-6 h) upon simulated gastrointestinal digestion (SGID) showed significant improvement in inhibition of both PL and CE. However, both the SGID hydrolysates showed similar extent of PL and CE inhibition and were further sequenced for peptide identification. Peptides MMML, FDML, HLPGRG from CwC and AAGF, MSNYF, FLWPEYGAL from CaC hydrolysates were predicted to be most active PL inhibitory peptides. Peptide LP found in both CwC and CaC hydrolysates was predicted as active CE inhibitor. Thus, CwC and CaC could be potential source of peptides with promising CE and PL inhibitory properties.
牛乳（CwC）和驼乳（CaC）水解产物分别使用 AlcalaseTM（CwCA 和 CaCA）和 Pronase-E（CwCP 和 CaCP）酶解 3 和 6 小时制备，并研究其抑制关键脂肪消化酶（即胰脂肪酶（PL）和胆固醇酯酶（CE））的潜力。结果表明，与 CwC 相比，CaC 水解产物对 PL 和 CE 的抑制作用更强。经过模拟胃肠道消化（SGID）后，有效的水解产物（CwCP-3 小时和 CaCA-6 小时）对 PL 和 CE 的抑制作用均显著提高。然而，SGID 水解产物对 PL 和 CE 的抑制程度相似，并且进一步进行了肽鉴定测序。来自 CwC 的 MMML、FDML 和 HLPGRG 肽和来自 CaC 水解产物的 AAGF、MSNYF 和 FLWPEYGAL 肽被预测为最具活性的 PL 抑制肽。在 CwC 和 CaC 水解产物中都发现的 LP 肽被预测为有效的 CE 抑制剂。因此，牛乳和驼乳可能是具有潜在的 CE 和 PL 抑制特性的肽的潜在来源。
