Wang Hengyan, Wang Songjun, Bai Huasong, Liu Tong, Wang Zhanzhong Z
Nourse Science Center for Pet Nutrition, Wuhu, 241200, China.
Vet Res Commun. 2025 Sep 18;49(6):314. doi: 10.1007/s11259-025-10886-7.
With increasing emphasis on companion animals, the nutrition and health of companion animals are attracting more and more concerns. In this work, digestive properties and in vitro bioavailability index (IVBAI) of four animal-derived proteins (casein, casein phosphopeptide, collagen and gelatin) in canine and feline food were evaluated using in vitro simulated digestion and cell absorption experiments. The results of high-performance liquid chromatography (HPLC) demonstrated that arginine, serine, lysine and glycine were produced during gastrointestinal digestion of four proteins. Meanwhile, the scale of peptides produced by digestion as determined by liquid-mass spectrometry (LC/MS) were mainly concentrated below 2000 Da. Digestion kinetic results indicated that casein phosphopeptide (CPP) and collagen (Col) could be digested faster by canine and feline compared with other proteins. It is worth noting from the intestinal absorption of Caco-2 cells that more than 13 amino acids could be absorbed, but the peptides digested could be not easily transported through cells. Finally, higher IVBAI in canine was confirmed to be Col and CPP, reaching respectively 25.60%±1.28% and 12.30%±0.61%, while it in feline was confirmed to be gelatin (Gel) and CPP, reaching 24.70%±1.23% and 19.30%±0.96%, respectively. These results indicated that Col and CPP could be more easily digested and absorbed in canine, while Gel and CPP could be more easily digested and absorbed in feline. These findings could provide important reference for the selection of animal proteins and nutritional enhancement of pet food.
随着对伴侣动物的重视程度不断提高，伴侣动物的营养与健康受到越来越多的关注。在本研究中，通过体外模拟消化和细胞吸收实验，评估了犬猫食品中四种动物源蛋白（酪蛋白、酪蛋白磷酸肽、胶原蛋白和明胶）的消化特性和体外生物利用度指数（IVBAI）。高效液相色谱（HPLC）结果表明，四种蛋白质在胃肠道消化过程中产生了精氨酸、丝氨酸、赖氨酸和甘氨酸。同时，液相质谱（LC/MS）测定的消化产生的肽段规模主要集中在2000 Da以下。消化动力学结果表明，与其他蛋白质相比，犬猫对酪蛋白磷酸肽（CPP）和胶原蛋白（Col）的消化速度更快。从Caco-2细胞的肠道吸收情况值得注意的是，有超过13种氨基酸可以被吸收，但消化产生的肽段不容易通过细胞转运。最后，证实犬的Col和CPP具有较高的IVBAI，分别达到25.60%±1.28%和12.30%±0.61%，而猫的则为明胶（Gel）和CPP，分别达到24.70%±1.23%和19.30%±0.96%。这些结果表明，Col和CPP在犬中更容易被消化吸收，而Gel和CPP在猫中更容易被消化吸收。这些发现可为宠物食品中动物蛋白的选择和营养强化提供重要参考。
