Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, China.
Department of Pediatrics, Liaocheng Third People's Hospital, Liaocheng, China.
Dev Neurosci. 2021;43(5):312-320. doi: 10.1159/000518352. Epub 2021 Jul 9.
Temporal lobe epilepsy (TLE) is the most common epilepsy in both adult and children. Some microRNAs (miRNAs) are abnormally expressed in neurological diseases. This study aimed to investigate the expression level and clinical significance of miR-148a-3p in TLE children and explore its effect on the biological viability of hippocampal neurons. The expression level of miR-148a-3p in the serum of TLE children was examined using quantitative real-time PCR. A receiver operating characteristic curve was plotted to determine the diagnostic accuracy of miR-148a-3p in TLE. Hippocampal neurons were cultured in magnesium-free medium to construct a TLE cell model. The effects of miR-148a-3p on hippocampal neuronal viability and apoptosis rate were detected by MTT and flow cytometry, respectively. miR-148a-3p was overexpressed and correlated with seizure frequency and febrile seizure (FS) history in TLE children. miR-148a-3p was of great value in the diagnosis of TLE, and it can be used to distinguish cases with FS history. Hippocampal neurons treated with magnesium-free medium were used as an in vitro model of TLE and showed significantly increased miR-148a-3p, decreased cell viability, and increased cell apoptosis, while these changes were eliminated markedly by miR-148a-3p knockdown. miR-148a-3p is overexpressed and associated with seizure frequency and FS history and serves as a novel diagnostic biomarker in TLE. In addition, the downregulation of miR-148a-3p exerts neuroprotective role by improving hippocampal neuronal cell viability. miR-148a-3p may provide new ideas for the treatment and diagnosis of TLE.
颞叶癫痫（TLE）是成人和儿童中最常见的癫痫类型。一些 microRNAs（miRNAs）在神经疾病中异常表达。本研究旨在探讨 TLE 患儿血清中 miR-148a-3p 的表达水平及其临床意义，并探讨其对海马神经元生物学活力的影响。采用实时定量 PCR 检测 TLE 患儿血清中 miR-148a-3p 的表达水平。绘制受试者工作特征曲线，以确定 miR-148a-3p 在 TLE 中的诊断准确性。采用无镁培养基培养海马神经元，构建 TLE 细胞模型。分别采用 MTT 和流式细胞术检测 miR-148a-3p 对海马神经元活力和凋亡率的影响。miR-148a-3p 在 TLE 患儿中与癫痫发作频率和热性惊厥（FS）病史相关。miR-148a-3p 对 TLE 的诊断具有重要价值，可用于区分有 FS 病史的病例。无镁培养基处理的海马神经元作为 TLE 的体外模型，miR-148a-3p 表达明显增加，细胞活力降低，细胞凋亡增加，而 miR-148a-3p 下调则显著消除了这些变化。miR-148a-3p 表达上调，与癫痫发作频率和 FS 病史相关，是 TLE 的新型诊断生物标志物。此外，miR-148a-3p 的下调通过提高海马神经元细胞活力发挥神经保护作用。miR-148a-3p 可能为 TLE 的治疗和诊断提供新的思路。
