Zhang Zhenghao, Wu Yong, Chen Jiangnan, Hu Fangqing, Chen Xuefang, Xu Wenfang
Clinical Laboratory, Affiliated Hospital of Shaoxing University (Shaoxing, China), Shaoxing, China.
Departnent of Gastroenterology, Affiliated Hospital of Shaoxing University, Shaoxing, China.
HIV Res Clin Pract. 2021 Jun;22(3):71-77. Epub 2021 Aug 4.
MicroRNA-155 (miR-155) regulates activation of T cells. However, its relationship with T-cell immune activation level in human immunodeficiency virus (HIV) patients remains unclear. We recruited 103 HIV-1 infected patients with combination antiretroviral therapy (cART) and 79 cART naïve patients. The miR-155 levels in circulatory T cells were detected by quantitative reverse transcription-PCR. T cell immune activation was detected by the expression of CD38 via flow cytometry. The levels of miR-155 in the total peripheral blood mononuclear cells, CD4+, and CD8+ T cells from HIV-1 patients were increased ( < 0.01). cART naïve patients exhibited much higher miR-155 levels in CD4 + and CD8+ T cells than patients with cART( < 0.01). The percentage of CD4 + CD38+ T and CD8 + CD38+ T cells was also increased in cART naïve patients ( < 0.01). MiR-155 level was positively related to immune activation of T cells. Our findings suggest that miR-155 levels in circulating T cells of HIV-1 patients are increased and associated with T cell activation.
微小RNA-155（miR-155）调节T细胞的激活。然而，其与人类免疫缺陷病毒（HIV）患者T细胞免疫激活水平的关系仍不清楚。我们招募了103名接受联合抗逆转录病毒治疗（cART）的HIV-1感染患者和79名未接受cART治疗的患者。通过定量逆转录PCR检测循环T细胞中的miR-155水平。通过流式细胞术检测CD38的表达来检测T细胞免疫激活。HIV-1患者外周血单个核细胞、CD4+和CD8+T细胞中的miR-155水平升高（<0.01）。未接受cART治疗的患者在CD4+和CD8+T细胞中的miR-155水平比接受cART治疗的患者高得多（<0.01）。未接受cART治疗的患者中CD4+CD38+T细胞和CD8+CD38+T细胞的百分比也增加了（<0.01）。miR-155水平与T细胞的免疫激活呈正相关。我们的研究结果表明，HIV-1患者循环T细胞中的miR-155水平升高，并与T细胞激活有关。
