Cuesta-Sancho Sara, Márquez-Ruiz Denisse, Illanes-Álvarez Francisco, Campaña-Gómez Irene, Martín-Aspas Andrés, Trujillo-Soto María Teresa, Romero Alberto, Galán Fátima, Rodríguez-Iglesias Manuel, Márquez-Coello Mercedes, Girón-González José-Antonio
Unidad de Enfermedades Infecciosas, Servicio de Medicina Interna, Facultad de Medicina, Hospital Universitario Puerta del Mar, Instituto de Investigación e Innovación en Ciencias Biomédicas de Cádiz (INiBICA), Universidad de Cádiz, Cádiz, Spain.
Servicio de Microbiología, Facultad de Medicina, Hospital Universitario Puerta del Mar, Instituto de Investigación e Innovación en Ciencias Biomédicas de Cádiz (INiBICA), Universidad de Cádiz, Cádiz, Spain.
Front Microbiol. 2023 Mar 2;14:1136718. doi: 10.3389/fmicb.2023.1136718. eCollection 2023.
To evaluate the serum expression of microRNAs (miRNAs) with ability to modulate the human immunodeficiency (HIV) replication or inflammatory status in people living with HIV (PLWH).
Forty healthy controls and two groups of PLWH were evaluated: (a) Group 1 ( = 30), patients with detectable viral load at inclusion, analyzed before receiving antiretroviral therapy (ART) and 12 months after initiating it; (b) Group 2 ( = 55), PLWH with prolonged undetectable viral load. Intestinal barrier disruption (I-FABP) and bacterial translocation (16S rDNA) markers, inflammatory markers such as interleukin (IL)-6 and sCD163, immune activation and expression of specific miRNAs were evaluated.
Serum concentrations of I-FABP, 16S rDNA, IL-6, sCD163 and activated T lymphocytes were increased in PLWH. Serum miR-34a was overexpressed at inclusion and remained elevated after ART. The expression of the remaining miRNAs that modulate HIV infectivity (miR-7, mir-29a, miR-150, and miR-223) was similar in PLWH and controls. Related to miRNAs implicated in inflammation (miR-21, miR-155, and miR-210), significant overexpression were observed in miR-21 and miR-210 levels in untreated PLWH, but levels were restored in those patients treated for a long period.
A sustained overexpression of miR-34a was detected even after prolonged HIV controlled replication. miR-21 and miR-210 can be considered new markers of inflammation with high sensitivity to its modifications.
评估具有调节人类免疫缺陷病毒（HIV）复制或炎症状态能力的微小RNA（miRNA）在HIV感染者（PLWH）中的血清表达情况。
对40名健康对照者和两组PLWH进行评估：（a）第1组（n = 30），纳入时病毒载量可检测到的患者，在接受抗逆转录病毒治疗（ART）前及开始治疗12个月后进行分析；（b）第2组（n = 55），病毒载量长期不可检测的PLWH。评估肠道屏障破坏（I-FABP）和细菌易位（16S rDNA）标志物、炎症标志物如白细胞介素（IL）-6和sCD163、免疫激活及特定miRNA的表达。
PLWH的血清I-FABP、16S rDNA、IL-6、sCD163和活化T淋巴细胞浓度升高。血清miR-34a在纳入时过表达，ART后仍保持升高。其余调节HIV感染性的miRNA（miR-7、mir-29a、miR-150和miR-223）在PLWH和对照中的表达相似。与炎症相关的miRNA（miR-21、miR-155和miR-210），未经治疗的PLWH中miR-21和miR-210水平显著过表达，但长期治疗的患者中这些水平恢复正常。
即使在HIV复制得到长期控制后，仍检测到miR-34a持续过表达。miR-21和miR-210可被视为对炎症变化高度敏感的新炎症标志物。
